磁分离仪分离磁性细菌的新方法  

Separation of magnetic bacteria by using a magnetic separator

作　　者：刘新星[1] 郭宁[1] 杨英杰[1] 梁万洁[1] 张剑[1] 

机构地区：[1]中南大学资源加工与生物工程学院,长沙410083

出　　处：《生物工程学报》2010年第2期270-275,共6页Chinese Journal of Biotechnology

基　　金：国家重点基础研究发展计划(973计划)(No.2004CB619201);国家自然科学基金(No.50774102)资助~~

摘　　要：磁性细菌胞内可以产生磁性颗粒,因此具有趋磁性,基于这种特性,利用磁分离的原理,本研究开发了一种磁性细菌分离仪,提供了一种分离磁性细菌的新方法。以氧化亚铁硫杆菌为例,使用磁性细菌分离仪进行分离,可以得到强磁菌和弱磁菌。利用透射电镜观察,强磁菌胞内磁性颗粒明显多于弱磁菌;半固体平板磁泳实验也表明强磁菌趋磁性明显强于弱磁菌。各项实验结果表明磁性细菌分离仪可以有效地分离磁性细菌,这是一种分离磁性细菌的新方法,将促进磁性细菌分离培养的研究。A magnetic separator was used to separate magnetic bacteria based on their magnetotactic characteristics. Acidithiobacillus ferrooxidans, a bacterium that could synthesize intra-cellular nanometer magnetic particles, was investigated as an example. Strong magnetic and weak magnetic cells were separated and collected. On average, the number of the magnetic particles present in the strong magnetic cells is more than that of the weak magnetic cells. Moreover, semisolid-plate magnetophoresis showed that the magnetotaxis of strong magnetic cells was stronger than the weak magnetic cells. These results suggest that the magnetic separator can be used to isolate the magnetic bacteria, which will facilitate the research of magnetic bacteria.

关 键 词：磁性细菌 细菌磁分离 磁性细菌分离仪 

分 类 号：Q937[生物学—微生物学]