High-coverage proteome analysis reveals the first insight of protein modification systems in the pathogenic spirochete Leptospira interrogans  

作　　者：Xing-Jun Cao Jie Dai Hao Xu Song Nie Xiao Chang Bao-Yu Hu Quan-Hu Sheng Lian-Shui Wang Zhi-Bin Ning Yi-Xue Li Xiao-Kui Guo Guo-Ping Zhao Rong Zeng 

机构地区：[1]Key Laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China [2]Graduate University of the Chinese Academy of Sci- ences, 320 Yue Yang Road, Shanghai 200031, China [3]Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China [4]State Key Laboratory for Disease and Health Genomics, Chinese National Human Genome Center at Shanghai, Zhangjiang High Tech Park, Shanghai 201203, China

出　　处：《Cell Research》2010年第2期197-210,共14页细胞研究（英文版）

摘　　要：Leptospirosis is a widespread zoonotic disease caused by pathogenic spirochetes of the genus Leptospira that infects humans and a wide range of animals. By combining computational prediction and high-accuracy tandem mass spectra, we revised the genome annotation of Leptospira interrogans serovar Lai, a free-living pathogenic spirochete responsible for leptospirosis, providing substantial peptide evidence for novel genes and new gene boundaries. Subsequently, we presented a high-coverage proteome analysis of protein expression and multiple posttranslational modifications （PTMs）. Approximately 64.3% of the predicted L. interrogans proteins were cataloged by detecting 2 540 proteins. Meanwhile, a profile of multiple PTMs was concurrently established, containing in total 32 phosphorylated, 46 acetylated and 155 methylated proteins. The PTM systems in the serovar Lai show unique features. Unique eukaryotic-like features of L. interrogans protein modifications were demonstrated in both phosphorylation and arginine methylation. This systematic analysis provides not only comprehensive information of high-coverage protein expression and multiple modifications in prokaryotes but also a view suggesting that the evolutionarily primitive L. interrogans shares significant similarities in protein modification systems with eukaryotes.

关 键 词：Leptospira interrogans posttranslational modification eukaryotic-like evolutionary conservation 

分 类 号：Q51[生物学—生物化学] S858.28[农业科学—临床兽医学]