Neurexin、Neuroligin基因在人类及鼠类动物肠神经系统的表达及意义  

Expressions of Neurexin and Neuroligin in the human and rodent enteric nervous system

作　　者：王健[1] 张文同[1] 孙大庆[2] 刘春喜[3] 李爱武[1] 

机构地区：[1]山东大学齐鲁医院小儿外科 [2]山东大学第二医院 [3]山东大学教育部、卫生部心血管重构与功能研究重点实验室

出　　处：《中华小儿外科杂志》2010年第2期132-136,共5页Chinese Journal of Pediatric Surgery

摘　　要：目的探讨Neurexin和Neuroligin基因在人类及鼠类动物肠神经系统（ENS）是否有表达，以及对ENS突触结构与功能的作用，探查其在先天性巨结肠各段的表达特征及意义。方法首先制备小鼠、大鼠、豚鼠、正常人体肠管肌间神经丛铺片，通过双重免疫荧光细胞染色，证明Neurexin、Neuroligin基因是否在肠神经系统表达，再采用免疫组织化学技术对20例先天性巨结肠狭窄段，移行段和扩张段肌间神经丛铺片标本染色，判断Neurexin、Neuroligin基因的表达情况。结果①Neurexin、Neuroligin蛋白在小鼠、大鼠、豚鼠、人体的肠肌间神经丛与神经细胞标记Hu在同一细胞表达；而Neurexin蛋白与突触前标记物synaptophysin，Neuroligin蛋白与突触后标记物PSED5也在同一神经组织出现；②Neurexin、Neuroligin蛋白在先天性巨结肠扩张段表达最多，移行段明显减少（P〈0．05），病变段无表达；同一组织Neurexin和Neuroligin的表达无明显差异（P〉0．05）。结论Neurexin、Neuroligin基因在鼠齿类动物及人体肠神经系统都有表达，不仅在胞体，而且在轴突、树突都有表达，Neurexin在突触前表达，Neuroligin在突触后表达。它们对肠神经系统突触的结构功能起重要作用，与一些胃、肠道动力性疾病有密切关系，是导致先天性巨结肠蠕动功能丧失的重要原因。Objective To detect the expressions of neurexin and neuroligin in the human and rodent enteric nervous system （ENS） and intestine tissues of the patients with Hischsprung＇s disease （HD）. Methods Longitudinal musele with adherent myenteric plexus （LMMP） were dissected from the intestines of mice, rats, guinea pigs and humans. The myenteric plexus was identified by staining neuron markers of neurexin-1 and Hu, prepsynaptic markers of neurexin-1 and synaptophysin and postsynaptic markers of neuroligin-1 and PSD95. The expressions of the Neurexin and Neurelogin in 20 HD cases were detected by immunohistochemistry. Results Neurexin-1, neuroligin-l-4 and Hu were detected in the some cells of the myenteric plexus of both rodents and humans. The expressions of Neurexin-1 and synaptophysin, and Neuroligin-1 and PSD95 were co-localized in the same tissues. The expressions.of neurexin and neuroligin were prominent in the ganglionic colonic segment of HD, they were decreased in the transitional segments, and negative in the aganglionic colonic segment. In the same HD tissue, no difference was found between neurexin and neuroligin expression. Conclusions Neurexin and Neuroligin are expressed in the ENS of both rodents and humans. Dysregulated expressions of Neurexin and Neuroligin may play important roles in the pathogenesis of HD.

关 键 词：肠神经系统 突触 HIRSCHSPRUNG病 

分 类 号：R346[医药卫生—基础医学] R726.5