活性氧诱导离体大鼠耳蜗Corti器毛细胞凋亡的观察  被引量:2

Observation of Reactive Oxygen Species on the Apoptosis of H_2O_2 Cochlear Hair Cell of Organ of Corti in Rat

在线阅读下载全文

作  者:原红艳[1] 张淑香[1] 李兴启[2] 李树华[3] 

机构地区:[1]天津武警医学院附属医院耳鼻咽喉科,天津300162 [2]中国人民解放军耳鼻咽喉科研究所 [3]沈阳军区总医院耳鼻咽喉科

出  处:《听力学及言语疾病杂志》2010年第2期164-166,共3页Journal of Audiology and Speech Pathology

摘  要:目的探讨氧自由基中毒后耳蜗毛细胞有无凋亡及其变化规律。方法选用新生1~5d龄SD大鼠24只,随机分为4组。每组6只(12耳):①无血清培养液组(H2O2浓度为零);②0.05mmol/LH2O2组;③0.1mmol/LH2O2组;①0.5mmol/LH2O2组。分离Cord器,并用尖刀按顶回、中回、底回将其分为3段,分别放人相应浓度的H2O2培养液中培养.培养结束后用丫啶橙/碘化丙啶双重染色技术检测并计数凋亡的毛细胞。结果不同浓度H2O2组均检测到凋亡毛细胞,外毛细胞(OHC)是H2O2攻击的主要靶细胞,而支持细胞无凋亡。底回毛细胞损伤明显重于顶回和中回,各回外毛细胞损伤明显高于内毛细胞;随H2O2浓度增加,各回凋亡细胞增加。结论本实验条件下,外源性H2O2可直接诱导离体培养大鼠耳蜗Corti器毛细胞凋亡。支持细胞无凋亡。Objective To observe the action of H2O2 on the hair cells in the organ of Corti of rats. Methods The culture technology of Corti in neonatal rat in vitro was used to establish the model of studying the injury of inner and outer hair cells caused by exogenous H2O2. 24 neonatal SD rats of 1-5 days were selected and divided into 4 groups with 6 in each group. There were 12 ears in each group:①serum- free medium;②0.05 mmol/L H2O2 ;③ 0.1 mmol/L H2O2 ;④0.5 mmol/L H2O2. Forty-eight hours after the tissues cultivation, the tissue was stained by AO and PI. The ratio of apoptotic cells in different groups was studied. Results Apoptosls cells were detected in groups of different concentration of H2O2 Outer hair cell were the main target attacked by H2O2. But not at apoptotic Sertoli cells . The loss of hair cells on the bottom, middle and parietal turn of basilar membranes varied as the injury to the bottom turn was more severe. The results suggest OFR could cause apoptosis of hair cells, and cochlea damage was dose-dependent in this way. Oonclusion Under the conditions of this experiment, exogenous H2O2 can cause the apoptosis of hair cells of the organ of Corti in rats, but not related to apoptotic Sertoli cells.

关 键 词:活性氧 H2O2 耳蜗 毛细胞 

分 类 号:R764.5[医药卫生—耳鼻咽喉科]

 

参考文献:

正在载入数据...

 

二级参考文献:

正在载入数据...

 

耦合文献:

正在载入数据...

 

引证文献:

正在载入数据...

 

二级引证文献:

正在载入数据...

 

同被引文献:

正在载入数据...

 

相关期刊文献:

正在载入数据...

相关的主题
相关的作者对象
相关的机构对象