HPLC-DAD/ELSD、UPLC-MS/MS分析复方五仁醇胶囊中皂苷类成分  

Analysis of Saponins in Compound Wurenchun Capsula by HPLC-DAD/ELSD and UPLC-MS/MS

作　　者：窦志华[1] 罗琳[2] 居文政[3] 丁安伟[4] 

机构地区：[1]南通市第三人民医院,南通226006 [2]南通大学,南通226006 [3]南京中医药大学附属医院,南京210029 [4]南京中医药大学,南京210029

出　　处：《药学与临床研究》2010年第1期38-41,共4页Pharmaceutical and Clinical Research

基　　金：江苏省中医药局中医药科学技术研究专项课题(编号:H05173)

摘　　要：目的:分析复方五仁醇胶囊中皂苷类成分。方法:分别采用HPLC-DAD/ELSD、U-PLC-MS/MS检测,通过与对照品色谱图比对及比较制剂和对照品提取离子流色谱图(EIC),并通过质谱图相关离子峰分析,鉴别制剂中的皂苷类成分。结果:HPLC-DAD/ELSD检测出三七皂苷R1、人参皂苷Rg1及人参皂苷Rb1,未检测到柴胡皂苷A和D;UPLC-MS/MS除检测到上述3种三七皂苷类成分外,还检测到柴胡皂苷D,但柴胡皂苷A仍未检测到。结论:复方五仁醇胶囊中存在三七和柴胡所含皂苷类成分。Objective： To analyze saponins in Compound Wurenchun Capsula. Methods： Saponins were identified by comparing the chromatograms detected by HPLC-DAD/ELSD and the extracted ion chromatograms （EIC） detected by UPLC-MS/MS of Compound Wurenchun Capsula with thoses of reference substances, correlated ion peaks in mass -spectrogram were analyzed at the same time. Results： Notoginsenoside R1, ginsenoside Rgl and ginsenoside Rbl were detected by HPLC-DAD/ELSD, but none of saikosaponin A or D was discovered through this method. Saikosaponin D was identified besides 3 saponins from Radix et rhizoma notoginseng by UPLC-MS/MS, but saikosaponin A was not detected yet. Conclusion： There are saponins from Radix et rhizoma notoginseng and Radix bupleuri in Compound Wurenehun Capsula.

关 键 词：高效液相色谱-二极管阵列/蒸发散射检测器 超高效液相色谱-串联质谱 人参皂苷 柴胡皂苷 复方五仁醇胶囊 

分 类 号：R284.1[医药卫生—中药学]