慢性乙型肝炎乙肝病毒血清标志物检测研究  

Study on determination of HBV serum markers of chronic hepatitis B

作　　者：陈宇宁[1] 陈华根[1] 许颖[2] 刘冰[1] 黄学斌[1] 宋强[1] 江俊[2] 

机构地区：[1]成都市新都区人民医院检验科,四川成都610500 [2]成都医学院第一附属医院检验科,四川成都610500

出　　处：《临床和实验医学杂志》2010年第3期175-177,共3页Journal of Clinical and Experimental Medicine

摘　　要：目的对慢性乙型肝炎患者进行乙肝病毒(HBV)血清标志物检测,分析结果,评价感染复制状况,选择适合临床防治需要、容易普及开展的标志物。方法采用酶联免疫吸附试验(ELISA)检测乙肝5项标志物[乙型肝炎病毒表面抗原(HBsAg)、乙型肝炎病毒表面抗体(抗-HBs)、乙型肝炎病毒e抗原(HBeAg)、乙型肝炎病毒e抗体(抗-HBe)、乙型肝炎病毒核心抗体(抗-HBc)]、前S1抗原、前S2抗原及前S2抗体,实时荧光定量检测HBV-DNA。286例慢性乙型肝炎患者分为HBeAg阳性组(126例)和HBeAg阴性组(160例),比较各组前S1抗原、前S2抗原、前S2抗体和HBV-DNA检出率。结果HBeAg阳性组,前S1抗原、前S2抗原、前S2抗体和HBV-DNA阳性率分别为:93.7%、77.0%、0和95.2%。HBeAg阴性组,前S1抗原、前S2抗原、前S2抗体和HBV-DNA阳性率分别为:55.0%、36.3%、0和58.1%。组内比较,HBeAg阳性组或HBeAg阴性组,前S1抗原与前S2抗原检出率差异具有统计学意义(P<0.05),前S1抗原与HBV-DNA检出率差异无统计学意义(P>0.05),前S2抗原与HBV-DNA检出率差异具有统计学意义(P<0.05)。组间相同项目比较,前S1抗原检出率、前S2抗原检出率、HBV-DNA检出率差异均具有统计学意义(P<0.05)。结论慢性乙型肝炎患者,前S1抗原与HBV-DNA具有良好的相关性,实验室常规开展乙肝5项和前S1抗原检测,可以判断乙肝病毒感染复制状况,为慢性乙型肝炎的预防、诊断和治疗提供实验依据。Objective To analyze the serum marker of patients with chronic hepatitis B virus infection and select the universal marker for clinic use.Methods HBsAg,anti-HBs,HBeAg,anti-HBe,anti-HBc,pre-S1 Ag,pre-S2 Ag and anti-pre-S2 Ab were measured by ELISA. HBV-DNA was determined by real time fluorescence quantitative polymerase chain reaction. The chronic HBV patients were divided into HBeAg positive group （126 cases） and HBeAg negative group （160 cases）. The detection rate of pre-S1 Ag,pre-S2 Ag,anti-pre-S2 Ab and HBV-DNA were compared to each others.Results The detection rates of pre-S1 Ag,pre-S2 Ag,anti-pre-S2 Ab and HBV-DNA in HBeAg positive group were 93.7%,77.0%,0 and 95.2% respectively. The detection rates of pre-S1 Ag,pre-S2 Ag,anti-pre-S2 Ab and HBV-DNA in HBeAg negative group were 55.0%,36.3%,0 and 58.1% respectively. There were intergroup differences in the detection rates of pre-S1 Ag,pre-S2 Ag and HBV-DNA between HBeAg positive group and HBeAg negative group （P〈0.05）. In addition,there were intragroup differences in the detection rates of both pre-S1 Ag,pre-S2 Ag in HBeAg positive group and HBeAg negative group （P〈0.05）.Conclusion The detection rate of pre-S1 Ag was closely correlated with HBV-DNA. The detections of HBsAg,anti-HBs,HBeAg,anti-HBe,anti-HBc and Pre-S1 Ag will provide experimental basis for the prevention,diagnosis and treatment of chronic hepatitis B patients.

关 键 词：乙型肝炎/慢性 标志物 检测 

分 类 号：R512.62[医药卫生—内科学] R457.1[医药卫生—临床医学]