氧化应激与大鼠增龄性骨量减少关系的初步探究  

The relationship between oxidative stress and age-related osteopenia in rats

作　　者：张育斌[1] 陈建庭[1] 钟招明[1] 张宇[1] 侯刚[1] 

机构地区：[1]南方医科大学南方医院脊柱骨病外科,广州510515

出　　处：《中国骨质疏松杂志》2010年第2期84-88,共5页Chinese Journal of Osteoporosis

基　　金：广东省自然基金资助课题(06024394)

摘　　要：目的探讨氧化应激与大鼠增龄性骨量减少之间的关系,为老年性骨质疏松症的临床干预提供理论依据。方法选用2、6及18月龄Wistar大鼠各20只,按月龄分为幼年、中年、老年3组,采用比色法检测血清中氧化指标晚期氧化蛋白产物(AOPP)、丙二醛(MDA)、一氧化氮(NO)含量和抗氧化指标超氧化物歧化酶(SOD)活力,并用双光子骨密度仪测定大鼠离体股骨骨密度值。结果(1)与幼年组、青年组相比,老年组骨密度显著降低(P<0.01),而幼年组与青年组之间无显著性差异(P>0.05)。(2)老年组与幼年组、青年组比较,AOPP、MDA含量升高(P<0.01),而幼年组与青年组之间无显著性差异(P>0.05),SOD活力则显示随年龄的增加而下降(P<0.05),各组之间NO含量差异无显著性(P>0.05);(3)股骨骨密度与AOPP含量之间呈负相关关系(r=-0.640;P<0.01);与MDA含量负相关(r=-0.421;P<0.01),与SOD活力正相关(r=0.483;P<0.01),与NO含量无相关性(r=-0.246;P>0.05)。结论随着大鼠年龄的增加,骨量逐渐减少,机体内的氧化应激水平逐渐增高,提示氧化应激与大鼠增龄性骨量减少关系密切。Objective To explore the relationship between oxidative stress and age-related osteopenia of rats, and to provide the theoretic basis for the intervention of age-related osteoporosis. Methods The colorimetry was adopted to measure the serum levels of AOPP, MDA, NO and SOD for Wistar rats at 2,6 and 18 months old （n = 20 in each group）. The BMD in femur was measured by Dual-energy X-ray absorptiometry（DEXA）. Results （ 1 ） The BMD in femur was decreased signifieantly in 18 months old rats compared with 2 and 6 months old groups （P 〈 0. 01 ） ,but it was not significantly different between 2 and 6 months old groups （P 〉 0.05 ）. （2）The serum levels of AOPP, MDA were increased significantly in 18 months old rats compared with 2 and 6 months old ones （ P 〈 0. 01 ） ,but they were not significantly different between 2 and 6 months old groups（ P 〉 0. 135 ）. The seerum level of NO was not significantly different among 3 groups（P 〉0.05）. The activity of SOD showed a decrease with aging（P 〈0.05）. （3） There was a negative correlation between AOPP and femur BMD levels （ r = - 0. 640 ; P 〈 0.01 ）. The same trend was observed between MDA and femur BMD （r = -0. 421 ;P 〈 0.01 ）. There was no correlation between NO levels and femur BMD levels（ r = - 0. 246 ; P 〉 0.05 ）. A positive correlation was observed between SOD and femur BMD levels （r = 0. 483 ;P 〈 0. 01 ）. Conclusions Increased level of oxidative stress is involved into age-related osteopenia in rats. Oxidative stress might play an important role in the pathophysiology of agerelated osteoporosis.

关 键 词：氧化应激 增龄性 大鼠 BMD AOPP MDA NO SOD 

分 类 号：R681[医药卫生—骨科学]