胎膜组织BMP-2及MMP-9的表达及与胎膜早破的关系  

The Relationship between the Expression of Bone Morphogenetic Protein-2 and Matrix Metalllopro Teinase-9 in Fetal Membrane and Premature Rupture of Membranes

作　　者：李庆姝[1] 漆洪波[1] 

机构地区：[1]重庆医科大学附属第一医院,重庆400016

出　　处：《实用妇产科杂志》2010年第2期139-142,I0001,共5页Journal of Practical Obstetrics and Gynecology

基　　金：重庆市自然科学基金项目(编号:03-8006);重庆市卫生局科研基金资金项目(编号:03-2-100)

摘　　要：目的:探讨胎膜组织骨形成蛋白-2(BMP-2)及基质金属蛋白酶-9(MMP-9)与胎膜早破(PROM)发生的关系。方法:选择足月临产产妇、足月PROM产妇、早产临产产妇、早产PROM产妇及足月未临产产妇(对照组)各18例,分娩后取破口处胎膜组织,采用免疫组织化学法染色,并使用病理影像多媒体图文操作系统对BMP-2、MMP-9阳性表达物的面积积分光密度(AIOD)进行半定量分析。结果:①胎膜组织中BMP-2和MMP-9阳性表达的AIOD在足月临产组、足月PROM组、早产临产组、早产PROM组均高于对照组,差异均有统计学意义(P<0.0071);②足月PROM组BMP-2和MMP-9高于足月临产组(P=0.0065,P=0.0069);早产PROM组BMP-2和MMP-9高于早产临产组(P=0.0069,P=0.0052);早产PROM组BMP-2和MMP-9高于足月PROM组(P=0.0037,P=0.0065);③5组胎膜组织中BMP-2和MMP-9的表达均呈明显正相关(rs=0.76159,P<0.0001)。结论:BMP-2和MMP-9与胎膜破裂的发生密切相关,胎膜组织中BMP-2与MMP-9的表达水平相关。Objective： To study the correlation between the bone morphogenetic proteins （BMP-2） and matrix metalloproteinase-9（MMP-9） in fetal membrane and premature rupture of membranes. Methods： 18 samples were selected separately from each of the following groups： puerpera with term pregnancy in labor, puerpera with term premature rupture of membranes（ PROM）, puerpera in preterm labor, puerpera with preterm PROM and puerpera with term pregnancy not in labor （control group）. After labor, fetal membranes in the rupture site were taken out. Immunobistochemistry and pathologic image multimedia operation system were adopted to semiquantitative analyze the area integrated optical density （AIOD） of the positive expression of BMP-2 and MMP-9. Results：（1）The AIOD of BMP-2 and MMP-9 positive expression in fetal membranes was higher in term pregnancy in labor group, term PROM group, preterm labor group and PROM group than that in control group. There was statistic difference （ P 〈 0.0071 ）. （2）The AIOD of BMP-2 and MMP-9 in term PROM group was higher than that in term pregnancy in labor group（ P = 0.0065, P = 0.0069）, which was higher in PROM group than that in preterm labor group （ P = 0.0069, P = O. 0052） and term PROM group P = 0.0037, P = 0.0065 ）, （3）There was obviously positive correlation between BMP-2 and MMP-9 expression in the fetal membranes in five groups. （ rs = 0.76159, P 〈 0.0001）. Conclusions.The expression of MMP-9 and BMP-2 is closely relevant to the repture of membranes. There is a correlation between BMP-2 and MMP-9 expression in fetal membranes.

关 键 词：骨形成蛋白-2 基质金属蛋白酶-9 胎膜早破 

分 类 号：R714.433[医药卫生—妇产科学]