低N胁迫下巴西橡胶实生苗叶片SPAD值、N素营养及农艺性状的变化  

The Changes and Relationship of Leaf SPAD Value,N Nutrition and Agronomic Traits in Leaves of Rubber Tree Seedlings under Low-N Stress

作　　者：周珺[1,2,3] 林位夫[1,3] 王军 曹建华[1,3] 蔡明道[1] 

机构地区：[1]中国热带农业科学研究院橡胶研究所,海南儋州571737 [2]海南大学农学院,海南儋州571737 [3]农业部橡胶树生物学重点开放实验室,海南儋州571737

出　　处：《亚热带植物科学》2010年第1期29-33,44,共6页Subtropical Plant Science

基　　金：海南省重点科技项目;橡胶所基本科研业务费专项;农业部农业行业科技专项(3-14)

摘　　要：对3个品种(GT1、RRIM600、PR107)巴西橡胶(Hevea brasiliensis)实生苗进行不同N素(0%N,50%N,100%N)处理,分析比较叶片SPAD值、N素营养和农艺性状的变化及其关系。结果表明:(1)缺N胁迫明显降低了叶片SPAD值、叶片N含量和硝酸还原酶活性,缩小了叶蓬距、叶面积,且影响幅度随胁迫强度的加大而增大;(2)不同品种橡胶树实生苗对缺N胁迫的响应程度不同,总体上GT1较为敏感,PR107次之,RRIM600较不敏感;(3)橡胶树实生苗叶片SPAD值、叶蓬距、叶面积与叶片N含量、硝酸还原酶活性之间均呈显著或极显著正相关。因此,有可能采用测定叶片SPAD值、叶蓬距和叶面积等简易方法判断橡胶树新生叶蓬的N素营养状况。Seedlings of three varieties （GT1, RRIM600, PR107） of Hevea brasiliensis were treated with low N （0%N, 50%N, 100%N） stress and the changes and their relationship of the leaf SPAD values, N nutrition and agronomic traits were studied. The results showed that： （1） low N stress significantly decreased the leaf SPAD value, leaf nitrogen content （LNC）, nitrate reductase activity （NRA） and reduced distance between leaf-umbrella （DBL）, leaf area （LA）. And these impact aggrandized as the stress intensity increased. （2） Responses to low-N stress were in different degrees between different varieties of rubber tree seedlings. On the whole, GT1 was most sensitive to stress, PR107 next, and RRLM600 was the least sensitive. （3） The SPAD value, DBL, LA were significantly positive correlated with LNC, NRA in the seedling leaves. Therefore, leaf SPAD value, DBL and LA would be shortcut measures to evaluate the nitrogen nutrient condition of rubber plant.

关 键 词：橡胶树 SPAD值 叶蓬距 叶面积 叶片N含量 硝酸还原酶活性 

分 类 号：S59[农业科学—作物学]