GLP-2对实验性梗阻性黄疸小肠上皮细胞MLCK表达的影响  

The effects of glucagon-like peptide-2 on the intestinal epithelium MLCK in rats with obstructive jaundice

作　　者：陈振勇[1] 冯贤松[1] 杨鹏[1] 周有生[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院普通外科,湖北武汉430022

出　　处：《中国普通外科杂志》2010年第2期151-154,共4页China Journal of General Surgery

摘　　要：目的探讨类高血糖素多肽-2(GLP-2)对大鼠实验性梗阻性黄疸小肠上皮细胞肌球蛋白轻链激酶(MLCK)的影响。方法建立梗阻性黄疸大鼠模型,10d后对照组给予0.01mmol/LPBS0.5mL,实验甲组给予250g/(kg.d),实验乙组给予125g/(kg.d)的GLP-2溶液0.5mL,分别皮下注射,均为每天2次,连续7d后处死。并设假手术组。检测末端回肠黏膜形态学变化;采用免疫组化和Western blots法检测MLCK的分布和表达,并利用图像分析系统对Westernblots图像结果进行定量分析。结果梗阻性黄疸时小肠黏膜萎缩明显,肠绒毛短而稀疏。外源性补充GLP-2后小肠绒毛上皮结构有所恢复;实验甲组平均肠绒毛高度、黏膜厚度和隐窝深度与对照组相比分别上升27.1%,26.4%和25.3%(均P<0.01)。梗阻性黄疸时对照组MLCK染色稀疏,分布不均。实验甲组的MLCK染色有所恢复,强阳性表达例数(8/10)较对照组(2/10)明显升高(P<0.05),实验乙组(4/10)没有变化(P>0.05)。Westernblot图像定量分析所得结果相同。实验甲组的绒毛高度的下降值与MLCK图像的吸光度的下降值呈正相关(r=0.62,P<0.01)。结论梗阻性黄疸时小肠黏膜明显萎缩,外源性补充GLP-2能增强小肠黏膜上皮MLCK的分布和表达,恢复肠黏膜上皮的形态。Objective To explore the effects of glucagon-like peptide-2 （GLP-2） on intestinal mucosa epithelium myosin light chain kinase （ MLCK ） of the rat with obstructive jaundice. Methods The obstructive jaundice models of rats were set up. At postoperative 10 d, the control group rats were subcutaneously injected with 0.5 mL PBS ; experiment group A with 250g/（ kg · d） GLP-2 （0.5 mL） , and experiment group B with 125 g/（ kg · d） GLP-2 （0. 5 mL） , b. i. d x 7 d,then all the rats were killed The villus height, mucosa thickness and crypt depth of the terminal ileum mucosa were detected. Immunohistochemistry and Western-blot were used to examine the distribution and expression of MLCK. Image analytical system and statistics software were used to analyze the results quantitately. Results The intestinal villi of the distal ileum mucosa were short and sparse in control group. Compared with control group, the average intestinal villus height, mucosa thickness and crypt depth were increased 27.8 % , 21.7 % and 25.4 % （ all P 〈 0. 01 ） in experiment group A. And the MLCK stainings appeared discontinuous and vague in control group, but they recovered somewhat in experiment group A. The values of strong positive expression were obviously higher in experiment group A than those in control group （8/10 vs. 2/10, P 〈0.05） and experiment group B（8/ 10 vs. 4/10 ,P 〉 0.05 ）. The same outcomes were obtained by quantitative analysing Western blot images. The degression of intestinal villus height and absorbance of MLCK images presented direct correlation. Conclusions The intestinal mucous membrane shows obvious atrophy in obstructive jaundice. Exogenous supplemented GLP-2 can enhance the quantity and distribution of MLCK in intestinal mucosa in obstructive jaurdice, and can restore the morphology of intestinal mucosa epithelium.

关 键 词：黄疸 阻塞性 类高血糖素多肽-2 肌球蛋白轻链激酶 大鼠 

分 类 号：R657.43[医药卫生—外科学]