三磷酸腺苷生物荧光法快速监测医务人员手清洁消毒效果  

RAPID MONITOR ON EFFECT OF MEDICAL STAFF HANDS CLEANING AND DISINFECTION BY ATP BIOLUMINESCENCE ASSAY

作　　者：柯雅娟[1] 许晨耘[1] 俞诗娃[1] 马秀苗[1] 

机构地区：[1]海南省人民医院,海口570311

出　　处：《中国消毒学杂志》2010年第2期161-162,共2页Chinese Journal of Disinfection

摘　　要：目的观察三磷酸腺苷生物荧光法快速监测医务人员手清洁消毒效果。方法采用棉拭涂抹采样法和三磷酸腺苷生物荧光法,对医务人员手消毒效果随机采样68例次,同时利用细菌培养计数法和ATP生物荧光法对卫生手清洁消毒后细菌含量进行检测。结果用三磷酸腺苷生物荧光法检测医务人员手消毒后68份样,合格率为89.71%;用细菌培养计数法检测医务人员手消毒后68份样,合格率为85.29%;两者方法检测结果基本一致,差异无统计学意义(P>0.05)。结论三磷酸腺苷生物荧光法能快速监测卫生手消毒效果,出结果快,省时省力。Objective To observe rapid monitor the effect of the medical staff hands cleaning and disinfection by ATP Bioluminescence Assay.Methods The cotton swabbing samples from 68 medical staff hands after disinfection randomly were detected with bacterial culture counting and ATP bioluminescence assay.Results With ATP bioluminescence assay,the pass rate of medical staff hands of 68 samples after disinfecting,was 89.71%.While with the bacterial culture counting method,the pass rate was 85.29%.The results of the two methods have no statistically significant difference（P〉0.05）.Conclusion ATP bioluminescence assay can be used to monitor the effects of health hand disinfection.It can show results quickly,saves time and manpower.

关 键 词：三磷酸腺苷生物荧光法 卫生手消毒 消毒效果 检测 

分 类 号：R187[医药卫生—流行病学]