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作 者:段晓东[1] 宋立新[1] 杨宏[1] 熊晓丽[1] 李威[1] 张杰[1]
机构地区:[1]北京工业大学北京市水质科学与水环境恢复工程重点实验室,北京100124
出 处:《北京工业大学学报》2010年第2期245-249,共5页Journal of Beijing University of Technology
基 金:国家“八六三”计划资助项目(2006AA06Z308)
摘 要:利用传统的细菌分离方法,从地下水生物除锰滤池中分离、纯化出1株细菌,经LBB(leucoberbelin blue)法定性检测其具有Mn2+氧化能力,利用Sherlock细菌鉴定系统初步确定其属于红球菌属(Rhodococcus sp).对红球菌Rhodococcus sp-1菌株的生物除锰能力和诱导特性研究结果表明,Rhodococcus sp-1菌株在达到第1个生长稳定期,细菌浓度2.3×108个/mL时,其Mn2+的去除量可达到35 mg/L,具有很强的Mn2+生物去除能力;同时Fe的存在对Rhodococcus sp-1 Mn2+氧化活性的表达具有诱导作用,在相同培养条件下,Fe3+的诱导性强于Fe2+,其诱导速度是Fe2+的3~4倍;有机Fe3+的诱导性强于无机Fe3+,其诱导速度是无机Fe3+的1.6~2倍.A strain bacteria had been separated, purified from the removal of manganese bio-fiher treated with groundwater by traditional microbiology method. The strain bacteria could oxidate Mn^2+ tested by the LBB method. It had been identified to belong to Rhodococcus sp. by the MIDI Sherlock bacteria identification system. Studied on the capacity and induction of Rhodococcus sp-1 biological manganese removal, the results shown: the removal amount of Mn^2 + was 35 mg/L when Rhodococcus sp-1 reached the first stabilization period, and it's order was 2.3 × 10^8 n/mL. It had the strong capacity of biological Mn^2+ removal. In addition Fe element could induct Rhodococcus sp-1 to oxidize Mn^2+ . Under the same conditions, Fe^3+ was stronger than Fe^2+ , the induction speed ratio was 3 -4:1 ; organic Fe^3+ was stronger than inorganic Fe^3+ , the induction speed ratio was 1.6 - 2: 1.
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