Nano-HA涂层与HA/CTS复合涂层抑制胶质母细胞瘤细胞活力作用的比较  

The inhibitory effect on glioblastoma cells viability by nano-HA coatings and HA/CTS coatings

作　　者：田昂[1] 王超[2] 管格非[2] 薛向欣[1] 吴安华[2] 王丽[1] 

机构地区：[1]东北大学材料与冶金学院,辽宁沈阳110004 [2]中国医科大学第一附属医院神经外科,辽宁沈阳110001

出　　处：《功能材料》2010年第2期300-303,共4页Journal of Functional Materials

基　　金：国家自然科学基金资助项目(50872019)

摘　　要：实验采用电化学沉积法在钛合金表面制备了纳米羟基磷灰石涂层(nHA)、纳米和微米级羟基磷灰石/壳聚糖复合涂层(nHA/CTS,mHA/CTS),并应用XRD、SEM和FTIR对涂层的理化特性进行了表征。然后将人脑胶质母细胞瘤细胞系U87(U87)与3种涂层共培养,并比较3种涂层诱导U87细胞凋亡的能力。通过MTT法细胞生长抑制实验检测以及电镜下膜层表面细胞形态观察,发现nHA膜层比nHA/CTS和mHA/CTS能更有效地抑制胶质瘤细胞的增殖,具有明显的体外抗肿瘤作用。In current study,nano-hydroxyapatite coatings（nHA）,nano-hydroxyapatite and micro-hydroxyapatite/chitosan complex films（nHA/CTS,mHA/CTS） were electrodeposited on the titanium substrate.The physical and chemical characteristics of the coatings were profiled using X-ray diffraction（XRD）,SEM and FTIR determination.After co-cultured with human glioblastoma multiforme cell line U87（U87）,the inhibitory effects of 3 kinds of surface layers on U87 cells viability were compared by MTT method and observation of the U87 cell morphology incubated on the coatings under SEM.The results showed that,among the 3 kinds of coatings,nHA coatings is more effective on inhibiting the proliferation of the U87 cells,and exhibits a potent anti-glioma capability in vitro.

关 键 词：羟基磷灰石 壳聚糖 胶质母细胞瘤 

分 类 号：TB333[一般工业技术—材料科学与工程]