大葱花药培养研究初报  

Primary Investigation in Anther Culture of Welsh Onion

作　　者：王文洁[1] 张国进[1] 周联东[1] 刘喜存[1] 郭玉霞[1] 

机构地区：[1]新乡市农业科学院,河南新乡453000

出　　处：《北方园艺》2010年第3期113-114,共2页Northern Horticulture

摘　　要：探索了大葱花药处于单核晚期的形态指标,并以B5为基本培养基,分别添加2,4-D(2.0～3.0 mg/L)、6-BA(0.5 mg/L)、谷胱甘肽(800 mg/L)等,对大葱花药进行了离体培养。结果表明:32℃热击处理有利于大葱花药诱导愈伤组织;培养集中添加6%浓度的蔗糖,有助于大葱愈伤组织的培养;大葱愈伤组织诱导率较低,且不易分化。The cytological observations of pollen in different developmental stages of welsh onion were undertook,135＋2, 4-D（2.0 = 3.0 mg/L） ＋ 6- BA（0. 5 mg/L） ＋ glutathione （800 mg/L） was used for callas formation. The results showed that pretreatment of anther after inoculation at 32℃ for 3 d and the medium with 6 % sucrose were favorable for callus formation. The callus induction rate of anther was low,and its differentiation was not easy.

关 键 词：大葱 花药培养 愈伤组织 

分 类 号：S633.103.6[农业科学—蔬菜学]