北海道黄杨茎尖组织培养初报  

Advances on the Establishment of Shoot-tip of Euonymus japonicus

作　　者：马少梅[1] 麻冬梅[1] 谢应忠[1] 许兴[1] 

机构地区：[1]宁夏大学农学院,宁夏银川750021

出　　处：《北方园艺》2010年第3期141-142,共2页Northern Horticulture

基　　金：国家"973"计划资助项目(2006CB100106)

摘　　要：以北海道黄杨的茎尖为材料,探讨了不同培养条件、生长素浓度等因子对建立茎尖再生体系的影响。结果表明:北海道黄杨茎尖诱导最适培养基为6-BA+0.5 mg/L+NAA 0.1mg/L,最适分化培养基为MS+6-BA 1.0 mg/L+NAA 0.2 mg/L,且在前10 d采用暗培养,之后采取光照培养。This paper used the shoot tips culture of Euonymus japonica as the materials,exploring the regeneration system of the shoot-tips culture was effected by the different conditions and their medium combination. The result showed that the most suitable induction medium for shoot-tip of Euonymus japonicus was 6-BA＋0. 5 mg/L＋NAA 0. 1 mg/L and the the optimal culture medium for culturing the multiplication of adventitious buds was MS＋6-BA1.0 mg/L＋NAA 0. 2 mg/L. The full dark conditions were adopted for about ten days,then gave the enough lights

关 键 词：北海道黄杨 茎尖 组织培养 再生体系 

分 类 号：S792.119[农业科学—林木遗传育种]