硅胶柱层析纯化胭脂虫红色素  

Purification of Cochineal Dye by Silica Gel Column Chromatography

作　　者：卢艳民[1,2] 郑华[1] 周梅村[2] 张弘[1] 

机构地区：[1]中国林业科学研究院资源昆虫研究所,昆明650224 [2]昆明理工大学化学工程学院,昆明650224

出　　处：《天然产物研究与开发》2009年第B10期383-385,共3页Natural Product Research and Development

基　　金：国家科技支撑计划（2006BAD06807）

摘　　要：研究了洗脱剂流速、负载量和硅胶初始含水量对硅胶柱层析分离纯化胭脂虫红色素的影响，得到了柱层析的优化工艺条件：硅胶初始含水量在10％-14％之间，洗脱剂流速为5ml/min，负载量为0．9g／100g硅胶。在优化条件下胭脂虫红色素经过硅胶层析后回收率在75％以上，纯度有明显改善，可达54．37％。This paper studied the effects of flow rate, sample load and initial water content in silica gel on the purification of cochineal dye. The results showed that the optimal conditions for the extraction were obtained as follows ： the initial water content in silica gel was in the range of 10%-14% ,flow rate 5 mL/min,sample load 0.9 g/100 g. After the process of silica gel column chromatography,the recovery of cochineal dye may be more than 75% and the content of carminic acid can reach 54.37%.

关 键 词：胭脂虫红色素 柱层析 硅胶 精制 

分 类 号：Q969.9[生物学—昆虫学] R284.1[医药卫生—中药学]