双波长系数倍率法测定净肝脂胶囊中山楂总黄酮的研究  

Study on Total Flavonoid in Jingganzhi Capsule by Dual-wavelength K-ratior Spectrophotometry

作　　者：励娜[1] 杨荣平[1] 张小梅[1] 王宾豪[1] 

机构地区：[1]重庆市中药研究院,重庆400065

出　　处：《天然产物研究与开发》2009年第B10期396-398,共3页Natural Product Research and Development

基　　金：重庆市科委项目（2004AAAS004）

摘　　要：为建立净肝脂胶囊中山楂总黄酮含量的测定方法，采用双波长系数倍率法测定波长和参比波长（500和400nm）。结果显示芦丁在15．04—75．20μg/mL范围内线性关系良好（r=0．9996），平均回收率为100．69％，RSD为1．67％（n=6）。本法简便快捷、定量准确，可作为净肝脂胶囊中总黄酮的含量测定方法。Dual-wavelength K-ratior spectrophotometry was applied to establish the method for measuring the content of total flavonoid in fruetus Crataegi at wavelength 500 and 400 nm. The concentration of rutin has a good linearity in the range of 15.04-75.20 μg/mL, r = 0.9996. The average recovery rate was 100.69% （ RSD = 1.67 %, n = 6）. This meth- od was simple, quick, accurate and suitable for the determination of total flavonoid in Jingganzhi capsule.

关 键 词：双波长系数倍率法 净肝脂胶囊 山楂总黄酮 含量测定 

分 类 号：R284.1[医药卫生—中药学] Q946.91[医药卫生—中医学]