制备型高效液相色谱法从秦艽中分离制备龙胆苦苷  

Preparation of Gentiopicroside from Gentiana straminea Maxim. by PHPLC

作　　者：张兴旺[1,2] 陶燕铎[1] 梅丽娟[1] 邵赟[1] 文怀秀[1] 赵晓辉[1] 

机构地区：[1]中国科学院西北高原生物研究所,西宁810008 [2]中国科学院研究生院,北京100049

出　　处：《天然产物研究与开发》2009年第B10期490-492,共3页Natural Product Research and Development

基　　金：国家科技攻关项目（0713351611）

摘　　要：利用制备高效液相色谱技术，从麻花秦艽根提取物中分离纯化得到高纯有效成分龙胆苦苷。对制备色谱的流动相组成，流速，进样量和检测波长等制备参数进行了优化，采用的制备色谱柱为Shim-pack PREP—ODS（K）（30mm×25cm，15／μm）；流动相为甲醇一水（V：V=1：2），流速为20mL／min；检测波长：270nm；进样体积为3mL，进样量为30mg。在20min的运行时间内，龙胆苦苷与其他成分得到很好的分离，产品纯度达98．2％。此方法简单快捷，可用于制备高纯度的龙胆苦苷。Preparative high performance liquid chromatography（ PHPLC） technique was used to separate and purify high purity of gentiopieroside from the crude extract of the root of Gentiana straminea Maxim. The flow rate of the mobile phase,injection volume and detection wave length were optimized. The optimum operation parameters were selected as follows ： a shim-pack PREP-ODS （K） （ 30 mm × 25 cm, 15 m ） with methanol-water = 1： 2 （ V： V） as the mobile phase at a f low rate of 20 mL/min, the detection wave length of 270 nm, and the injection volume of 3 mL at the concentration of 10 mg/mL. Gentiopicroside can be obtained in 20 min run time,and the purity of the product was detected by HPLC method. The result showed the purity of gentiopicroside was 98.2%. This method is brief and rapid and can be applied to the preparation of high purity of gentiopicroside from Gentiana straminea Maxim.

关 键 词：秦艽 制备型高效液相色谱 龙胆苦苷 

分 类 号：O657.72[理学—分析化学] TS202.3[理学—化学]