菊花晚熟红高效再生体系的建立及其玻璃化苗逆转  

Establishment of Efficient Regeneration System and Reversion of Vitrification of Wanshuhong

作　　者：马丽华[1] 张龙霞[1] 于鑫[1] 乔增杰[1] 田保华[1] 王一娟 裴雁曦[1] 

机构地区：[1]山西大学生命科学与技术学院,山西太原030006

出　　处：《山西大学学报（自然科学版）》2009年第A02期79-84,共6页Journal of Shanxi University(Natural Science Edition)

基　　金：山西省科技攻关计划项目（20090311014）

摘　　要：利用菊花名品晚熟红叶片作为外植体通过直接诱导不定芽的方法建立高效稳定的再生体系，为进一步的基因转化奠定基础，并对组织培养过程中出现的严重玻璃化现象进行了逆转研究．结果发现在设定的30种不同激素浓度配比的分化培养基中MS＋6-BA0．5mg／L＋NAA2．0mg／L的直接分化率最高．在组培苗玻璃化逆转的研究中，设计了7种方案，其中改变激素浓度和琼脂浓度处理的逆转效果较好．An efficient and stable regeneration system was established by directly inducing adventitious shoot from leaves of Wanshuhong for further genetic transformation, moreover, the reversion of serious vitrification during tissue culture was studied. Setting 30 groups of differentiation medium which contain different concentration of 6-BA and NAA,the results indicated that MS＋6-BA0.5 mg/Lq-NAA2. 0 mg/L is the optimum group. To reverse vitrification of shoot during tissue culture,this study set 7 groups of which differring the concentration of hormone and agar show the better effect.

关 键 词：菊花 晚熟红 再生体系 玻璃化苗 逆转 

分 类 号：Q942[生物学—植物学]