慢性焦虑应激大鼠行为学的变化及中药丹栀逍遥散的干预作用  

Ethological changes in rats with chronic stress-induced anxiety and interventional effect of Danzhixiaoyao Powder

作　　者：李宁[1] 唐启盛[2] 赵瑞珍[2] 勾圣乐[1] 

机构地区：[1]北京中医药大学,北京100029 [2]北京中医药大学第三附属医院

出　　处：《北京中医药大学学报》2009年第12期826-829,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.30772804)

摘　　要：目的以慢性焦虑应激大鼠为载体,从行为学的角度探讨中药丹栀逍遥散的干预作用。方法采用不确定性空瓶刺激的方法建立焦虑应激大鼠模型,观察大鼠空瓶刺激时探究、修饰和攻击行为的变化。应激结束后以国际公认的高架十字迷宫试验对其行为学进行评价,观察中药的干预作用。结果应激后大鼠处于易激惹的状态,表现出频繁的修饰和攻击行为。经高架十字迷宫测试发现应激后的大鼠较少进入和停留于开放臂中,倾向于探究封闭臂,且在封闭臂的停留时间延长,运动活力降低,而中药干预后大鼠的修饰和攻击行为明显减少,总体活动力增加,且探究开放臂的次数和停留时间增多。结论不确定性空瓶刺激作为一种慢性心理应激模型,从病因学上较好模拟了焦虑症的发病状态,中药丹栀逍遥散具有明显抗焦虑的作用。Objective To investigate the interventional effect of Danzhixiaoyao Powder from the aspect of ethology in rats with chronic stress-induced anxiety.Methods The rat model of chronic stress-induced anxiety was established by using the stimulation of uncertainty empty bottle.The changes of exploratory, grooming and attack behaviors were observed when rats were stimulated by empty bottle.After the stress the ethology of rats were reviewed by applying international elevated plus-maze（EPM） test.The interventional effect of Danzhxiaoyao Powder was observed.Results The rats were irritable after the stress and had frequency grooming and attack behaviors.The result of EPM test showed that the rats were not willing to enter or stay in the open arm,but tended to explore the block arm and stay in there for a longer time.The motor activity of the rats decreased.After the intervene of Danzhixiaoyao Powder the grooming and attack behaviors of rats were reduced significantly,the total motor activity increased,and exploring times and stay time in the open arm were improved.Conclusion The model of chronic psychological stress by the stimulation of uncertainty empty bottle simulates well the anxiety in rats etiologically.Danzhxiaoyao Powder has a significant anti-anxiety effect.

关 键 词：焦虑模型 行为学 慢性情绪应激 丹栀逍遥散 大鼠 

分 类 号：R285.5[医药卫生—中药学]