蝉拟青霉原生质体形成与再生  

Formation and Regeneration of Protoplasts from Paecilomyces Cicadae

作　　者：张永军[1] 刘爱英[1] 梁宗琦[1] 

机构地区：[1]贵州大学虫生真菌研究室

出　　处：《贵州农业科学》1998年第5期1-4,共4页Guizhou Agricultural Sciences

基　　金：贵州省基金

摘　　要：比较了不同酶液、菌丝生长培养基、渗透稳定剂、酶解温度及菌龄等因素对原生质体形成的影响。用１５％的溶壁酶、菌丝生长培养基为淀粉琼脂培养基（ＳＴＳ），０６ＭＫＣｌ为渗透稳定剂，温度２８℃，菌龄１６ｈ，可从所试菌株的菌丝获得大量的原生质体（４．０５×１０６个／ｍｌ）。０．６ＭＮａＣｌ为稳定液，黄豆粉培养基上原生质体再生率可达７９７％。对原生质体释放和再生的形态学过程进行了描述和显微摄影。The effects of such factors as enzyme systems, media for mycelia growth, osmotic stabilizers, different temperatures and age of mycelium on the formation of the mycelia protoplasts were studied.High yield of protoplasts were obtained from 16 hours mycelia of Paecilomyces cicadae by using 1.5% lyvallzyme as enzyme system, starch agar medium as mycelia; growth medium and 0.6% KCl as osmotic stabilizer at the temperature of 28℃. When 0.6M NaCl was used as stabilizer, about 7.97% of protoplasts regenerated and formed colonies on soybean medium (SOS). The morphological processes of protoplasts and regeneration were illustrated by microphotograph.

关 键 词：蝉拟青霉 原生质体 真菌 

分 类 号：S432.44[农业科学—植物病理学] Q949.32[农业科学—农业昆虫与害虫防治]