两种β_2受体激动剂快速免疫检测方法  

Development of Immunoassay Methods for two β 2 Agonists

作　　者：陈继明[1] 韩正康[1] 龚晓明[1] 陆承平[1] 杜念兴[1] 

机构地区：[1]南京农业大学动物医学院

出　　处：《中国兽医科技》1998年第9期5-8,共4页Chinese Journal of Veterinary Science and Technology

基　　金：国家自然科学基金

摘　　要：分别以偶氮方法和碳化二亚胺（ＥＤＣ）方法将β２受体激动剂克伦特罗（ＣＬ）和塞曼特罗（ＣＩＭ）连接到载体蛋白上，并以连接物免疫家兔，分别制备兔抗ＣＬ和ＣＩＭ抗血清，经间接ＥＬＩＳＡ和间接抑制ＥＬＩＳＡ检测抗血清效价和特异性，并在此基础上建立了ＣＬ和ＣＩＭ快速免疫检测方法。２种抗血清工作浓度分别为１∶１００００和１∶５０００。ＣＬ和ＣＩＭ免疫检测范围分别为１．９ｎｇ～１５．６μｇ和０．４７７ｎｇ～１．８５μｇ。２种β２受体激动剂快速免疫检测方法的建立为监督畜牧生产上滥用ＣＬ和ＣＩＭ，检测肉品ＣＬ和ＣＩＭ残留提供了一种简便、灵敏、特异的手段。Two β 2 agonists, Clenbuerol (CL) and Cimaterol (CIM) were conjugated with carrier proteins by diazotization and EDC respectively. These conjugates were used to immunize rabbits to prepare anti CL and anti CIM sera. The titers and the specificities of the sera were detected by indirect ELISA and inhibitory indirect ELISA. Then the sera were used to build up immunoassay methods for the two kinds of β 2 agonists. The work dilutions of two kinds of sera were 1∶10 000 and 1∶5000 respectively and the sensitivites of detection ranged from 1.9 ng to 15.6 μg and from 0.477 ng to 1.85 μg respectively. These immunoassay methods could be used to examine the overdue usage of CL and CIM in animal productions and to detect the residue of CL and CIM in meat products.

关 键 词：Β2受体激动剂 克伦特罗 塞曼特罗 免疫检测 

分 类 号：S859.793[农业科学—临床兽医学]