尿NMP22检测在膀胱癌诊断中的应用  

Detected Urinary NMP22 Applited for Diagnosis of Blader Cancer

作　　者：朱小军[1] 袁亚光[1] 

机构地区：[1]内蒙古医学院附属医院泌尿外科,内蒙古呼和浩特010050

出　　处：《内蒙古医学杂志》2010年第1期9-12,共4页Inner Mongolia Medical Journal

摘　　要：目的:探讨测定尿中NMP 22的含量在膀胱癌诊断中的应用价值。方法:应用ELISA法检测29例膀胱癌术前患者(实验组)、15例膀胱癌术后复测患者(对照组),20例泌尿系良性疾病患者(对照组),15例健康志愿者(对照组),尿样中NMP22的含量。同时行尿脱落细胞学检查并进行对比。结果:实验组29例术前尿NMP22含量中位数为27.63 u/ml;15例术后尿NMP22含量中位数为7.62 u/ml;20例泌尿系良性疾病中位数为7.25 u/ml;15例健康志愿者的中位数为2.17 u/ml。膀胱癌患者尿NMP22的平均水平均显著高于其他3组的NMP22的平均水平,实验组与其他3组之间的差异具有统计学意义(P<0.01)。以10 u/ml为诊断参考值时,NMP22诊断膀胱癌的敏感度为82.76%,特异度为65%。而尿脱落细胞学检查的敏感度仅为34.48%,特异度为100%。15例术后复测患者的NMP22平均水平显著低于术前(P<0.01),且实验组NMP22水平与肿瘤分级密切相关、与临床分期无关。结论:尿NMP22含量的测定可作为膀胱癌诊断和监测的一个辅助指标,且具有简单、无创伤、敏感度高、可定量分析等优点。Objective：To study the clinical significance of the NMP22 for diagnosis and surveillance of blader cancer.Methods：NMP22 content in urine was determined by ELISA（enzyme-linked immunosorbent assay）in conrtol group comprised 29 patients before the resection of bladder cancer,in group 1 included 15 patients after the resection of bladder cancer,in group 2 included 20 patients with benign urinary tract conditions,and in group 3 included 15 cases normal healthy.Urine cytology were also carried out for control group at the same time.Results：Median NMP22 value for control group was 27.63 u/ml,for group 1 was 7.62 u/ml,for group 2 was 7.35 u/ml,and for group 3 was 2.17 u/ml.NMP22 in control group was higher than the mean value of other three groups with statistical significance（P0.01）.When the cut off was set 10 u/ml,the sensitivety of NMP22 was 82.76%with a specificity of 65%.The sensitivety of urine cytology was 34.48%with a specificity of 100%.Median NMP22 value for group 1 was significantly lower than preoperative value（P0.01）.Close relationship were be noted between urinary NMP22 values and grade of the bladder cancer,have nothing to do with stage.Conclusion： NMP22 in urine may be as a promising tumor marker for the diagnosis and surveillance of bladder cancer,and it is simple,noninvasive,sensitive and quantitative.

关 键 词：尿 NMP22 膀胱肿瘤 移行细胞癌 

分 类 号：R345.9[医药卫生—基础医学] R737.14