晚期糖基化终产物对大鼠血管平滑肌细胞增殖影响的实验研究  

Experimental Study on Advanced Glycation end Products Induced Proliferation of Rat's Vascular Smooth Muscle Cells in Vitro

作　　者：齐佳昕[1] 张雅杰[1] 王麟[2] 

机构地区：[1]内蒙古民族大学第二附属医院心内科内分泌科,内蒙古牙克石022150 [2]哈尔滨医科大学大庆校区生理教研室,黑龙江大庆163319

出　　处：《内蒙古医学杂志》2010年第1期18-21,共4页Inner Mongolia Medical Journal

摘　　要：目的:研究晚期糖基化终产物(Advanced Glycation end Products,AGEs)对体外培养大鼠主动脉血管平滑肌细胞(Vascular Smooth Muscle Cells,VSMCs)增殖的影响。方法:组织贴块法培养大鼠VSMCs,给予不同浓度AGE-牛血清白蛋白(AGE-BSA)连续处理VSMCs 168 h(7 d),每24 h采用噻唑蓝(MTT)法检测细胞增殖,绘制细胞生长曲线,计算细胞增殖促进率。结果:80 mg/L终浓度的AGE-BSA在培养72、961、20 h,10、20、40 mg/L终浓度的AGE-BSA在培养96、120、1441、68 h均可显著促进细胞增殖(P<0.05);40 mg/L终浓度的AGE-BSA在培养96 h时细胞增殖促进率最大(72.1%)。结论:AGEs具有促进VSMCs增殖作用,这一作用在一定范围内具有剂量依赖性。Objective：To investigate the role of Advanced Glycation end products（AGEs） on proliferation of rat＇s Vascular Smooth Muscle Cells（VSMCs）.Methods： Rat＇s VSMCs were cultured in vitro by the tissue explants adherent method,then treated with AGE-BSA of different concentrations for 168 hours constantly.VSMCs proliferation was tested by MTT every 24 hours,cells growth curve was drawn and cells proliferation promotion rate were calculated.Results： AGE-BSA of 80 mg/L concentration treatment for 72、96、120 hours as well as AGE-BSA of 10、20、40 mg/L concentration treatment for 96、120、144、168 hours can significantly promote the proliferation of VSMCs （P0.05） the highest proliferation promotion rate appeared at AGE-BSA of 40 mg/L concentration treatment for 96 hours（72.1%）.Conclusion： In a certain extent,AGEs can concentration dependently promotes the proliferation of VSMCs.

关 键 词：晚期糖基化终产物 血管平滑肌细胞 细胞培养 动脉粥样硬化 

分 类 号：R543.5[医药卫生—心血管疾病]