黑骨藤中总黄酮含量的测定  

Determination of the Total Flavonoids in Periploca forrestii Schltr

作　　者：景小楠[1] 钟菡[1] 张宏[1,2] 于树华[1,2] 

机构地区：[1]四川师范大学生命科学学院,四川成都610066 [2]四川师范大学植物资源应用与开发研究所,四川成都610066

出　　处：《四川师范大学学报（自然科学版）》2010年第1期89-92,共4页Journal of Sichuan Normal University（Natural Science）

基　　金：国家自然科学基金(30870257)资助项目

摘　　要：对采自不同产地、不同年份黑骨藤的总黄酮含量进行了测定.以芦丁为对照品,检测波长510 nm,绘制标准曲线,采用紫外分光光度法测定总黄酮的相对百分含量.芦丁对照品在2.065～103.250 mg.L-1范围内有良好的线性关系(r=0.999 90),平均回收率为98.03%,RSD值为1.40%.采用分光光度法测定黑骨藤的总黄酮含量操作简便、结果可靠,为评价黑骨藤的质量提供了依据.Determination of the total flavonoids in Periploca forrestii Schltr of different areas and different years has been performed in this paper.The percentage of total flavonoids was determined by ultraviolet spectrophotometry at 510 nm based on the standard curve,with rutin as controlled sample.There was good linearity（r=0.999 27） during the range of 2.065~103.250 mg·L-1 for rutin.The average recovery was 98.03% and the RSD was 1.40%.The content of total flavonoids in Periploca forrestii Schltr of different areas and different years was measured.The ultraviolet spectrophotometry method is simple and reliable to measure the content of total flavonoids in Periploca forrestii Schltr.The results provide the evidence for evaluating the quality of Periploca forrestii Schltr.

关 键 词：黑骨藤 总黄酮 紫外分光光度法 

分 类 号：R917[医药卫生—药物分析学] S816.17[医药卫生—药学]