mtDNA SNPs研究方法进展及法医学应用现状  

Advances of mtDNA SNPs technologies and its application in forensic medicine

作　　者：何琼[1] 刘超[2] 陈玲[1] 王慧君[1] 

机构地区：[1]南方医科大学法医学系,广州510515 [2]广州市刑事科学技术研究所,510030

出　　处：《国际遗传学杂志》2010年第1期32-37,58,共7页International Journal of Genetics

基　　金：基金项目：公安部重点科技攻关项目（20034421401）

摘　　要：SNPs是不同个体基因组DNA序列中单个核苷酸的差异所致序列多态性，是继RFLP和STR标记发展起来的新一代遗传标记系统。线粒体是细胞内的惟一核外基因组，对于核DNA分型失败样本、降解样本、毛发（干）、指甲、骨骼等生物检材具有良好的实际应用价值。本文对目前线粒体SNPs分型最常用的技术及方法，如MALDI-TOFMS、SNaPshot TM、焦磷酸测序、IlluminaGoldenGate测序、lllumina Infinium测序、Taqman探针、SNPlex、SNPstream、分子信标技术及其在法医学实际应用进行了综述。Single nucleotide polymorphism （SNP） is defined as a single nucleotide difference giving rise to sequence polymorphism among individuals, as a new genetic marker following RFLP and microsatellite polymorphisms. Mitochondrial DNA （mtDNA） is a unique gcnomic DNA outside cell nucleus, and is very useful in the situation of analyzing failed samples of STR genotyping, degraded samples, hair （shafts） , and finger nails in the long run. Here, new mtDNA SNP genotypc technologies, including MALDI-TOF MS, SNaPshotTM assay, Illumina GoldenGate assay, Illumina Infinium assay, Taqman, SNPlex, SNPstream, molecular beacons, and their application in forensic medicine were reviewed.

关 键 词：线粒体DNA SNPS 法医学 

分 类 号：D9[政治法律—法学]