The expression of Bcl-2 and Bax produced by sub-chronic intoxication with the cyanotoxin Microcystin-LR  

微囊藻毒素-LR亚慢性染毒对Bcl-2和Bax表达的影响(英文)

作　　者：Zhijian Hu Hua Chen Jinfa Xue Xiaoyang Zhang Xishun Shi Hong Zou 

机构地区：[1]Public Health School, Fujian Medical University, Fuzhou 350004, China [2]Fuzhou Health Supervision Office, Fuzhou Health Authority, Fuzhou 350001, China

出　　处：《The Chinese-German Journal of Clinical Oncology》2010年第2期68-72,共5页中德临床肿瘤学杂志（英文版）

基　　金：Supported by grants from the Natural Science Foundation of Fujian Province (No C0710019);the Science & Technology Program of Fuzhou (No 2006S-G24)

摘　　要：Objective:We explored the role and molecular mechanism of Microcystin-LR(MC-LR) in hepatocarcinogenesis.Methods:The two-stage-medium-term theory was applied to the establishment of the animal model.The promoting effect of MC-LR on liver tumor was evaluated with the Albert γ-GT methods.During the tumor-promoting course,the effects of MC-LR on the regulation and expression of the Bcl-2 and Bax genes were studied with immunohistochemical technique and RT-PCR.Results:MCLR could enhance the positive reaction of Albert γ-GT(GGT),a preneoplasm marker.The positive reaction rate of GGT in diethylnitrosamine(DEN) + MC group was significantly higher than that in the DEN control group.The protein and RNA expression of Bcl-2 was significantly increased by MC-LR,and that the protein expression of Bax was decreased simultaneously.Conclusion:These results indicate that the MC-LR can inhabit apoptosis through the Bcl-2 and Bax genes.Therefore,we conclude that the expression change of Bcl-2 and Bax genes possibly plays an important role in the promotion of liver tumor by MC-LR.

关 键 词：MICROCYSTIN-LR Albert γ-GT BCL-2 BAX liver cancer 

分 类 号：Q949.23[生物学—植物学] Q255