Oxaliplatin and teniposide can inhibit the proliferation and induce the apoptosis of gastric cancer cell line BGC-823 synergistically  被引量:1

奥沙利铂联合替尼泊甙对胃癌BGC-823细胞的生长抑制和诱导凋亡的协同作用(英文)

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作  者:Wang Ma Ming Gao Wei He Qingxia Fan 

机构地区:[1]Department of Oncology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China

出  处:《The Chinese-German Journal of Clinical Oncology》2010年第3期149-152,共4页中德临床肿瘤学杂志(英文版)

摘  要:Objective:The aim of this study was to investigate the synergistic effects of oxaliplatin and teniposide on proliferation and apoptosis of gastric cancer cell line BGC-823.Methods:MTT assay was carried to examine the inhibition rate of oxaliplatin and teniposide on gastric cancer cell line BGC-823 with various concentrations separately and associatively.The apoptosis rate of BGC-823 cells under the treatment of oxaliplatin or/and teniposide was examined by flow cytometry.The expression level of livin, an apoptosis-associated protein, was explored by western blot.Results:Oxaliplatin or teniposide could remarkably inhibit the BGC-823 gastric cancer cell growth with a dose-response manner, separately and associatively.The inhibition rate of oxaliplatin combined with teniposide on BGC-823 cells was higher than that of single oxaliplatin or single teniposide(P < 0.05), with 0.46 as combination index(CI) value.The apoptosis rates of cells treated by oxaliplatin for 12 h, 24 h and 48 h were 6.13%, 13.86% and 21.48%, respectively, while which of teniposide were 4.60%, 10.72%, 17.07%.But when the two medicines were carried associatively, the apoptosis rates for 12 h, 24 h and 48 h were 11.73%, 24.14% and 44.75%, respectively.Western blot showed that the expression level of livin was more down-regulated when cells were treated by oxaliplatin + teniposide than by oxaliplatin singly.Conclusion:The combination of oxaliplatin and teniposide can exert a synergistic effect on gastric cancer cell BGC-823.

关 键 词:gastric cancer OXALIPLATIN TENIPOSIDE APOPTOSIS flow cytometry 

分 类 号:Q255[生物学—细胞生物学] TQ236[化学工程—有机化工]

 

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