钩藤碱对甲基苯丙胺条件性位置偏爱大鼠AMPA受体蛋白改变的影响  

The effect of rhynchophylline on AMPA receptors expression in methamphetamine dependent rats

作　　者：林晓亮[1] 汤伟[1] 陈文倩[1] 翁建霖[2] 莫志贤[1] 

机构地区：[1]南方医科大学中医药学院,广州510515 [2]香港浸会大学生物学系

出　　处：《中华行为医学与脑科学杂志》2010年第2期104-107,共4页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金资助项目（30828033）

摘　　要：目的观察甲基苯丙胺成瘾大鼠伏隔核及下丘脑中AMPA受体表达的改变及钩藤碱对其的干预作用。方法SPF级雄性SD大鼠分为空白对照组、甲基苯丙胺模型组、钩藤碱低剂量组及钩藤碱高剂量组，每组8只。采用条件性位置偏爱（CPP）实验建立甲基苯丙胺成瘾大鼠模型，采用蛋白免疫印迹（Western blotting）方法观察大鼠伏隔核及下丘脑中GluR2／3亚基表达的改变。各组大鼠在伴药箱中的停留时间采用独立样本t检验，蛋白条带光密度分析采用单因素方差分析。结果与空白对照组[给药后伴药箱中停留时间为（383．00±38．20）s]比较，甲基苯丙胺能引起大鼠位置偏爱[给药后伴药箱中停留时间为（536．20±57．49）s]，低剂量（30mg／kg）及高剂量（60mg／kg）钩藤碱[给药后伴药箱中停留时间分别为（299.80±15．96）s、（189．40±59．02）s]均能消除甲基苯丙胺诱导的大鼠位置偏爱。与空白对照组[平均灰度值的比值分别为（0.54±0．04）INT·mm^2、（0．70±0．04）INT·mm^2]比较，甲基苯丙胺模型组[平均灰度值的比值为（0．89±0．03）INT·mm^2]及钩藤碱低剂量组[平均灰度值的比值为（0．93±0．03）INT·mm^2]大鼠伏隔核内GluR2／3亚基蛋白表达显著上调（P〈0．01），下丘脑内GluR2／3亚基蛋白[平均灰度值的比值分别为（0．53±0．03）INT·mm^2、（0．52±0．04）INT·mm^2]表达则显著下调（P〈0．01）。钩藤碱高剂量组大鼠伏隔核及下丘脑内GluR2／3亚基蛋白[平均灰度值的比值分别为（0．57±0．06）INT·mm^2、（0．65±0．01）INT·mm^2]表达与空白对照组无差异（P〉0．05）。结论甲基苯丙胺CPP大鼠伏隔核内GluR2／3亚基蛋白表达增多，下丘脑内GluR2／3亚基蛋白表达减低，高剂量钩藤碱可将上述改变恢复至正常水平。Objective To study changes of AMPA receptors expression in nucleus aceumbens and hypothalamus of methamphetamine dependent rats, and the therapeutical effect of rhynchophylline. Methods SPF male rats were randomly divided into normal control group, model group of methamphetamine, low dose of rhynchophylline group and high dose of rhynchophylline group （ n = 8 in each group）. Experiment of conditioned place preference（CPP） was used to build the model of methamphetamine dependent rats. Western blotting was used to examine the changes of GluR2/3 subunits expression. The time of staying in drug-paired compartment of rats was used independent-samples t test to gather statistics, and the photodensity of proteinnm strap was used One-Way ANOVA to gather statistics. Results Compare with rats in normal control group （ the time of staying in drug-paired compartment of rats was （383.00 ± 38.20） s）, the rats produced CPP after treated with methamphetamine （ the time of staying in drug-paired compartment of rats was （ 536.20± 57.49 ） s ）, and low （ 30 mg/kg） and high （60mg/kg） dose of rhynchophylline （the time of staying in drug-paired compartment of rats were （299.80 ± 15.96） s and （189.40± 59.02）s） both could eliminate CPP effect. Compare with rats in normal control group （ the ratio of value of average gray scale were （0.54± 0.04） INT · mm^2 and （0.70 ± 0.04 ） INT · mm^2 ）, GluR2/3 subunits expression in nucleus accumbens increased significantly in model group（ the ratio of value of average gray scale was （0.89 ±0. 03） INT · mm^2 ） and low dose of rhynchophylline group （ the ratio of value of average gray scale was （0.93±0. 03 ）INT ·mm^2, P 〈 0. 01 ）, which decreased significantly in hypothalamus（ the ratio of value of average gray scale were （0.53 ± 0.03 ） INT ·mm^2 and （0.52 ± 0.02 ） INT . mm^2, P 〈 0.01 ）. But GluR2/3 subunits expression in nucleus accumbens and hypothalamus of rats in high dose of rhynchophylline group

关 键 词：钩藤碱 甲基苯丙胺 条件性位置偏爱 AMPA受体 

分 类 号：R285.5[医药卫生—中药学]