异氟醚对老年小鼠空间参考记忆和脑细胞凋亡及Caspase-3表达的影响  

Effects of isoflurane on spatial reference memory and changes of Caspase-3 expression and apoptosis in brain of aged mice

作　　者：柳垂亮 李玉娟[2] 陈郡兴[3] 李国才 谢晓澜 

机构地区：[1]广州中医药大学第二附属医院麻醉科,广州510120 [2]中山大学第二附属医院麻醉科 [3]广州医学院第三附属医院麻醉科

出　　处：《中华行为医学与脑科学杂志》2010年第2期108-111,共4页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家青年科学基金项目（30700787/C03030301）;广东省医学科学技术研究基金（B2007083）

摘　　要：目的观察异氟醚对老年小鼠空间学习记忆能力及其大脑Caspase-3蛋白表达、细胞凋亡的影响。方法24只16月龄C57BIM6老年小鼠分层随机分为异氟醚组（Iso，n=12）和对照组（Con，n=12）。Iso组使用1.0％异氟醚行麻醉暴露4h／d，连续2d；Con组同期只使用30％的氧气和70％氮气混合气。在麻醉暴露后，采用Morris水迷宫检测小鼠空间学习记忆能力，再分别使用免疫荧光染色法及TUNEL法检测小鼠大脑皮质区（CX）、海马CA1区和齿状回（DG）的Caspase-3蛋白表达及神经细胞凋亡的变化。结果在Morris水迷宫的定位航行测试中，2组间逃避潜伏期的变化差异无显著性（F=0．007，P=1．235），但在空间探索实验测试中，Iso组在原平台所在象限（靶象限）停留时间[（34．5±5．0）％]明显短于Con组[（45．1±4．9）％]，差异具有显著性（P〈0．01）。测试过程中2组小鼠寻找平台游泳的速度差异无显著性（F=1．537，P=0．241）。免疫荧光染色检测结果显示，DG、CA1及CX区均可见Caspase-3阳性细胞，然而2组间的单位面积的阳性细胞数（阳性密度）比较差异无显著性（P〉0．05）。TUNEL法检测到极少量阳性凋亡细胞。结论重复使用1％异氟醚麻醉暴露可损害老年小鼠的空间参考记忆能力，但无明显影响大脑Caspase-3蛋白表达及细胞凋亡。Objective To investigate the effect of isoflurane on the spatial learning and memory in aged mice,and whether this is associated with the changes of Caspase-3 expression and apoptosis in brain. Methods Twenty-four C57BL/6 aged mice （16 months） were randomly divided into isoflurane treatment group （Iso Group, n= 12） and sham control group （ Con Group, n= 12）. Mice in Iso group were exposed to 1% isoflurane in carrying gas of 30% oxygen,balance nitrogen in a warmed,humidified chamber for 4 h per day for 2 days. For Con group, animals were treated at the same condition with only carrying gas. After anesthetic exposures, behavioral testing was performed using the Morris water maze（ MWM）, and then changes of Caspase-3 expression and apoptosis in hippocampus CA1 ,dentate gyms（DG） and cortex（CX） in brain were determined by using immunofluorecence staining and TUNEL staining. Results In hidden-platform training of MWM,the mean escape latency to platform showed no significant difference between the two groups （F = 0. 007, P = 1. 235 ）, but the mice in Iso group showed obviously impaired retention of memory by spending more percentage of time swimming in the probe quadrant as compared to the controlanimals in the probe test （ （ 34.5 ±5.0） % vs （45.1 ±4.9） %, P 〈 0.01 ）. There was no significant difference in average swimming speed during the MWM testing trials between the two groups （F = 1. 537, P = 0. ：241 ）. A few Caspase-3 and apoptosis positive cells were found in hippocampus CA1, DG and CX regions, while no difference was found in the density of positive cells between the two groups （P〉 0. 05 ）. Conclusion 1% isoflurane repeatedly exposure significantly impaires the spatial reference memory in aged mouses, however does not significantly change the expression of caspase-3 and apoptosis in brain.

关 键 词：异氟烷 参考记忆 上鼠 老年 Caspase-3 

分 类 号：R965[医药卫生—药理学]