四物合剂对顺铂损伤小鼠卵巢的保护作用  

Protective effects of Siwuheji on cisplatin induced ovarian dysfunction in mice

作　　者：孙丽萍[1] 王继峰[1] 牛建昭[1] 赵丕文[1] 郝庆秀[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《中国中药杂志》2010年第4期481-484,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30772849);高等学校学科创新引智计划(B07007);博士点基金项目(20060026019)

摘　　要：目的:观察四物合剂对顺铂损伤小鼠卵巢的保护作用,并对其作用机制进行探讨。方法:小鼠腹腔注射顺铂3.0 mg.kg-1.d-1,连续7 d,并灌饲四物合剂14 d,观察小鼠动情周期和卵巢、子宫的系数变化,血清中雌性激素水平及卵巢形态。结果:注射顺铂3 d始,模型小鼠动情周期停滞于间期,至13 d时,灌饲四物合剂小鼠部分开始恢复动情周期变化;顺铂模型组小鼠卵巢系数显著低于对照组,四物合剂各组小鼠的卵巢系数与对照组比较无差异,与模型组比较有统计学差异;模型组血清E2和P水平较对照组有降低,但无统计学意义,四物合剂大剂量组E2较模型组升高,小剂量组P较模型组升高,但也均无统计学意义;模型组FSH/LH比值较对照组明显升高,四物合剂中、小剂量组可恢复到正常水平,与模型组比较变化显著;卵巢切片显示模型小鼠卵巢中的各级发育卵泡数量减少,而闭锁卵泡数量增加,四物合剂中剂量组可见多个发育卵泡。结论:顺铂可诱导小鼠卵巢损伤,使其功能下降,四物合剂可改善顺铂诱导的卵巢功能损伤。Objective： To evaluate the protective effects of Siwuheji on cisplatin-induced ovarian impairing and explore its possible mechanisms in mice.Method： The mice received cisplatin ip（7 mg kg-1） for 7 days,while fed with Siwuheji for 14 days.The changes of estrous cycle,ovary and uterus coefficient,levels of estrogen in serum and morphology of ovary were recorded.Result： From the third day of cisplatin injection,the estrous cycle of the model group stagnated in interphase.On the thirteenth day,part of the mice fed with Siquheji started with change of estrous cycle.The ovary coefficient of the model group was less than the control group,the experimental groups were no differences compared with the control group and had significant differences with the model group.The levels of E2 and P of model group were lower than the control group,but no statistical significance.The level of E2 with high-dose Siwuheji group was higher than model group,and the level of P of the low-dose group was higher than model group,but both no statistical significances.The FSH / LH ratio of model group was significantly higher than the control group,low-dose group and medium-dose group of Siwuheji resumed to normal levels,and had a significantly change compared with the model group.Ovarian biopsy of model group showed the decrease in the number of developing follicle at all levels,and increase in the number of Atresia of ovarian.There were more growing follicte in the large,medium-dose group.Conclusion： Cisplatin can induce mice ovarian injury and functional decline,on the other hand,Siwuheji can improve cisplatin-induced ovarian dysfunction.

关 键 词：四物合剂 顺铂 卵巢 雌激素 卵泡刺激素 

分 类 号：R285.5[医药卫生—中药学]