姜黄素对人肝癌细胞Sk-hep-1抗癌作用的研究  

Anticancer activities of curcumin on human hepatocarcinoma cell line Sk-hep-1

作　　者：王伟章[1] 张碧鱼[2] 陈宏远[1] 张黎[1] 

机构地区：[1]广东药学院,广东广州510006 [2]中山大学珠海校区基础教学中心,广东珠海519082

出　　处：《中国中药杂志》2010年第4期485-488,共4页China Journal of Chinese Materia Medica

基　　金：广东省医学科研基金项目(A2009318)

摘　　要：目的:研究姜黄素对人肝癌细胞Sk-hep-1体外抗癌作用,探讨其抗癌作用的分子机制。方法:应用MTT法、DA-PI核染色法和细胞周期分析法,检测姜黄素对Sk-hep-1细胞生长和凋亡的影响,并通过RT-PCR方法检测姜黄素对Sk-hep-1细胞抗凋亡基因(Survivin和Bcl-xL)和耐药基因(DRG2和MDR1)mRNA表达的影响。结果:姜黄素抑制Sk-hep-1细胞增殖呈量效关系。姜黄素处理组肝癌细胞Sk-hep-1,HepG2和Hep3B细胞发生不同程度的凋亡,正常肝细胞Chang’s liver无明显凋亡。姜黄素处理组Sk-hep-1细胞周期发生变化,G0/G1或G2/M期细胞比例增多。姜黄素处理组Sk-hep-1细胞耐药基因MDR1 mRNA水平显著下降,而抗凋亡基因的表达无明显变化。结论:姜黄素能够抑制Sk-hep-1细胞增殖,并诱导其凋亡,推测可能是通过下调MDR1 mRNA的表达而起作用。To study the anticancer activities of curcumin on human hepatocarcinoma cell line Sk-hep-1 and its related molecular mechanism which has not been elucidated.In the present study,we showed that curcumin inhibited proliferation of Sk-hep-1 cells in a dose-dependent manner through MTT assay.The effect of curcumin on apoptosis in Sk-hep-1 cells was investigated by DAPI staining and the various apoptosis was observed in hepatocarcinoma cell lines Sk-hep-1,HepG2 and Hep3B,but not in normal liver cell line Chang＇s liver with curcumin treatment.Cell cycle analysis results showed that curcumin treatment resulted in dramatic accumulation of Sk-hep-1 cells at the G0/G1 or G2/M phase.The effect of curcumin on the expression of anti-apoptosis genes（Survivin and Bcl-xL） and drug resistance genes（DRG2 and MDR1） was studied by reverse transcription-polymerase chain reaction（RT-PCR）.The expression of MDR1 mRNA was significantly decreased in Sk-hep-1 cells treated with curcumin,while no alterations in the amount of DRG2 and anti-apoptosis genes＇ mRNA levels were found.These results indicate that curcumin is able to inhibit proliferation and induce apoptosis in Sk-hep-1 cells and it may cause by down-regulating the expression of MDR1 mRNA.

关 键 词：姜黄素 肝癌细胞株Sk-hep-1 细胞凋亡 MDR1基因 

分 类 号：R285[医药卫生—中药学]