不同种质来源柴胡地上部分的HPLC比较研究  

Comparison on HPLC analysis of aerial part of Bupleurums from different germplasm resources

作　　者：耿茂林[1] 宋萍萍[1] 徐增莱[1] 王年鹤[1] 

机构地区：[1]江苏省中国科学院植物研究所江苏省药用植物研究开发中心,江苏南京210014

出　　处：《中国中药杂志》2010年第5期574-578,共5页China Journal of Chinese Materia Medica

摘　　要：目的:对不同种质来源柴胡的地上部分进行HPLC比较研究。方法:21份不同种质来源柴胡在相同条件下播种,以二年生地上部分为样品进行HPLC分析,对得到的色谱图进行聚类分析。结果:不同种质来源的柴胡地上部分HPLC图有明显差异,表明柴胡具有丰富的种质多样性。结论:HPLC比较法较全面地反映不同种质来源柴胡地上部分的差异,可用于药材质量控制,在柴胡属植物分类学上有一定的指导意义。Objective： To investigate ingredient differences of the aerial part of Bupleurums from different germplasm resources by HPLC. Method： Twenty-one different germplasm resources were sown and cuhured at the same conditions and the aerial parts of the biennial plants were analyzed by HPLC. Result： The chromatograms were established and the data were analyzed by Hierarchical Cluster Analysis to compare the similarity of the samples. The results showed that ingredients from various germplasm resources were significantly different, which indicated the germplasm diversity of Bupleurum. Conclusion： The method can be used for comparison of the ingredient differences and quality control, the results provide important references for classification of Bupleurum species.

关 键 词：柴胡 不同种质来源 地上部分 高效液相色谱 聚类分析 

分 类 号：S567.79[农业科学—中草药栽培]