黄芩苷对非白念珠菌生物膜抑制作用的研究  

In vitro activity of baicalin against non-albicans Candida biofilms

作　　者：汪长中[1] 程惠娟[1] 张雄飞[1] 徐胜利[1] 官妍[1] 俞丽华[1] 云云[1] 

机构地区：[1]安徽中医学院中西医结合临床学院,安徽合肥230038

出　　处：《中国中药杂志》2010年第5期639-641,共3页China Journal of Chinese Materia Medica

基　　金：安徽省自然科学基金项目(090413092);安徽省高校自然科学研究项目(KJ2008B223)

摘　　要：目的:研究黄芩苷对光滑念珠菌、近平滑念珠菌、克柔念珠菌、季也蒙念珠菌等非白念珠菌生物膜的影响。方法:96孔微量培养板上构建4种非白念珠菌生物膜;微量稀释法检测黄芩苷对4种非白念珠菌最低抑菌浓度(MIC);XTT减低法评价黄芩苷对4种非白念珠菌生物膜SMIC及菌细胞黏附性的影响。结果:4种非白念珠菌在96孔板中可形成成熟的生物膜;黄芩苷对4种非白念珠菌MIC分别是125,250,125,62.5 mg.L-1;黄芩苷对4种非白念珠菌生物膜的SMIC50分别是(1 000,500,125,250 mg.L-1;SMIC80均大于或等于1 000 mg.L-1。黄芩苷对4种非白念珠菌的黏附有一定的抑制效应。结论:黄芩苷对近平滑念珠菌、克柔念珠菌、季也蒙念珠菌生物膜有较强的抑制作用。Objective ： To investigate the effects of baicalin against Candida glabrata, C. parapsilosis, C. krusei and C. guilli- ermondii biofihns. Method： 96-well microtitre plates were used to set up the biofilms ; microdilution method was applied to detect minimal inhibitory concentration（MIC） of baicalin for the four non-albians Candida, and XTT reduction assay was adopted to determine sessile minimal inhibitory concentration（ SMIC ） of baicalin against the four isolates and to detect the effects on adhesion of the fungal cells. Result： MICs of baicalin for the four non-albians Candida cells were 125,250, 125, 62. 5 mg· L^-1 , respectively. The four nonalbians Candida could form mature biofihns on 96-well microtitre plates. SMIC50 of baicalin for the four isolates were 〉 1 000, 500, 125, 250 mg · L^-1 , respectively. SMIC80 for the four isolates were greater than br equal to 1 000 mg· L^-1. Baicalin showed potent inhibitory effects on adhesion of the four non-albians Candida cells. Conclusion： Baicalin displays substantial inhibitory effects on C. parapsilosis, C. krusei and C. guilliermondii biofilm.

关 键 词：黄芩苷 非白念珠菌 光滑念珠菌 近平滑念珠菌 克柔念珠菌 季也蒙念珠菌 生物膜 

分 类 号：R285[医药卫生—中药学]