对C57BL/6小鼠超排效果的研究  

Study on the effect of superovulation in C57BL/6 mice

作　　者：关文怡[1] 曹授俊[1] 齐军喆[1] 

机构地区：[1]北京农业职业学院畜牧兽医系,北京102442

出　　处：《四川畜牧兽医》2010年第2期26-27,共2页Sichuan Animal & Veterinary Sciences

摘　　要：本文以近交系C57BL/6小鼠为实验对象,研究注射不同剂量的PMSG和hCG对小鼠超排效果的影响。取C57BL/6小鼠各30只,按照注射剂量不同分为A、B、C三组,每组10只,A组注射PMSG2.5IU,HCG2.5IU,B组注射PMSG5.0IU,HCG5.0IU,C组注射PMSG7.5IU,HCG7.5IU。每只小鼠腹腔注射PMSG,间隔48h后分别注射HCG进行超数排卵,再与性成熟同系公鼠合笼,次日早上检查阴道栓,有栓雌鼠用颈椎脱臼法处死。在实体显微镜下由输卵管膨大部冲卵,收集卵母细胞置于盛有M2培养液的表面皿中检查计数,分析超排效果。结果表明,C57BL/6小鼠B组的平均取卵数极显著高于A组的平均取卵数(P<0.05);B组的平均取卵数显著高于C组的平均取卵数(P<0.05);C组与A组的平均取卵数差异不显著(P>0.05)。To study the effects of PMSG and hCG on superovulation in mice. Thirty C57BL/6 mice were divided into 3 groups, which received PMSG 2.5 IU＋HCG 2.5 IU in group A, PMSG 5.0IU＋HCG 5.0IU in group B, and PMSG 7.5IU＋HCG 7.5IU in group C. After intraperitoneal injection of PMSG, HCG were injected 48h later, The female mice were combined with mature male mice in one cage, and were checked for embolus the next morning. Oocytes were washed out from ampullar segment of oviduct, and were counted in M2 culture fluid. The average number of washing out oocytes in group B was more than that of group A（ P〈0.05 ）, and no significant difference between group A and group C （ P〉0.05 ）.

关 键 词：C57BL/6小鼠 超数排卵 研究 

分 类 号：S814.8[农业科学—畜牧学]