乙型肝炎免疫球蛋白聚氰基丙烯酸正丁酯纳米粒的体外跨膜转运试验  

In Vitro Membrane Transport of Hepatitis B Immunoglobulin Polybutylcyanoacrylate Nanoparticles

作　　者：彭忠田[1,2] 谭德明[1] 黄顺玲[1] 朱平安[1,3] 刘菲[1] 

机构地区：[1]中南大学湘雅医院,长沙市410008 [2]南华大学附属第一医院,衡阳市421001 [3]深圳市第七人民医院,深圳市518081

出　　处：《中国药房》2010年第9期799-801,共3页China Pharmacy

基　　金：湖南省长沙市科学技术局基金资助项目(K051155-72)

摘　　要：目的:探讨纳米化对乙型肝炎免疫球蛋白(HBIG)的细胞内浓度和渗透率的影响。方法:取HBIG聚氰基丙烯酸正丁酯纳米粒(HBIG-PBCA-NP),以QSG7701细胞为体外细胞模型,首先采用MTT法检测HBIG-PBCA-NP和HBIG在试验浓度下对细胞的毒性作用;再分别对细胞加入HBIG-PBCA-NP和HBIG处理后HBIG的胞内浓度和HBIG渗透率进行不同时间点的对比考察。结果:HBIG-PBCA-NP和HBIG在试验浓度下对细胞无毒性作用;HBIG-PBCA-NP组的HBIG胞内浓度和HBIG渗透率在各时间点均明显高于HBIG组(P<0.05或P<0.01)。结论:HBIG纳米化能增强HBIG的细胞渗透能力,提高HBIG的胞内浓度。OBJECTIVE： To investigate the effect of nanominiaturization on concentration and penetration of hepatitis B im- mune globulin （HBIG） in cells. METHODS： HBIG polybutylcyano-acrylate nanoparticles （HBIG-PBCA-NP） were prepared and QSG7701 cell lines were used as in vitro cell model. First, MTT method was carried out to determine HBIG-PBCA-NP and HBIG＇ s toxic action to cells under the test concentration. Then HBIG-PBCA-NP and HBIG were added into cells to compare intracellular concentrations of HBIG and penetrability of HBIG at different time. RESULTS： It was found that HBIG-PBCA-NP and HBIG had no toxic action to cells and all of determined cellular HBIG concentration in the HBIG-PBCA-NP group was higher than in HBIG group （P〈0.05 or P〈0.01）. CONCLUSION： The results indicate that penetrability and concentration of HBIG across the cellular membrane has been improved by nanominiaturization.

关 键 词：乙型肝炎免疫球蛋白 纳米粒 QSG7701细胞 转运 

分 类 号：R965[医药卫生—药理学] R975.5[医药卫生—药学]