麦红吸浆虫滞育不同时期幼虫蛋白质双向电泳分析  

Two-dimensional electrophoresis of proteins in the pre-diapause,diapause and post-diapause larvae of wheat blossommidge,Sitodiplosis mosellana (Gehin)

作　　者：成卫宁[1] 李怡萍[1] 杨杰[1] 许向利[1] 仵均祥[1] 

机构地区：[1]西北农林科技大学应用昆虫学重点实验室,陕西杨凌712100

出　　处：《植物保护学报》2010年第1期7-11,共5页Journal of Plant Protection

基　　金：陕西省自然科学基金(2009JM3003);教育部重点项目(105165);国家自然科学基金(30370936)

摘　　要：为筛选与麦红吸浆虫滞育相关的蛋白,采用双向电泳及图像分析技术分析了麦红吸浆虫滞育前、后和滞育期的蛋白质图谱及其变化。结果表明,在滞育前幼虫的双向电泳图谱上平均可检测到蛋白质点约300个,其它时期约275个,其中多数分布在分子量8.0～50.0kD、等电点4.0～9.0的区域。在所检测到的蛋白质点中,26个在滞育前特异表达;3个在滞育前缺失,其它阶段表达;2个在滞育后200天特异表达;3个在滞育前和滞育解除后表达,滞育期缺失;34个在所有时期均表达,但各时期的表达量存在显著差异。In order to screen diapause associated protein of wheat blossom midge, Sitodiplosis moseUana （Gehin）, two-dimensional gel eleetrophoresis （2-DE） and image analysis system were used to analyze differential expression of proteins extracted from pre-diapause, diapause and post-diapause larvae of wheat blossom midge. On 2-DE gels, about 300 protein spots were detected in the extracts of pre-diapause larvae and 275 for those in each of other stages, and the majority of these spots were located in the area 8.0 - 50.0 kD and pI 4.0 - 9.0. Among detected proteins, 26 were unique in pre-diapanse, 3 were expressed during diapause and post-diapause but missing in pre-diapause, 2 were unique in diapause 200 days, 3 were expressed during pre-diapause and post-diapause but absent in diapause stages, 34 were expressed in all stages but showed different expression levels among larvae in different stages.

关 键 词：麦红吸浆虫 滞育 蛋白质 双向电泳 

分 类 号：S435.122[农业科学—农业昆虫与害虫防治]