壳寡糖诱导烟草防御酶系活性变化及PR-1a基因表达研究  

Changes of defensive enzymes and PR-1a gene expression of tobacco induced by chito-oligosaccharides

作　　者：商文静[1] 吴云锋[1] 赵小明[2] 杜昱光[2] 商鸿生[1] 

机构地区：[1]西北农林科技大学植物保护学院陕西省农业分子生物学重点实验室,杨凌712100 [2]中国科学院大连化学物理研究所,大连116023

出　　处：《植物病理学报》2010年第1期99-102,共4页Acta Phytopathologica Sinica

基　　金：教育部长江学者和创新团队发展计划(200558);高等学校学科创新引智计划(B07049);西北农林科技大学博士启动基金资助

摘　　要：The activity change of defensive enzymes and PR-1a gene expression of tobacco (Nicotiana tabacum) seedling induced by chito-oligosaccharides were studied. The results showed that high level systemic acquired resistance (SAR) was expressed in tobacco plants treated with chito-oligosaccharides solution at the concentration of 50 μg/mL. PAL activity increased greatly with 2 peaks, the activity of SOD decreased initially followed by an increase with higher increment, and the activity of POD peaked early followed by a gentle fall in chito-oligosaccharide treated plants. The PR-1a gene was strongly expressed in tobacco due to systemic acquired resistance induced by chito-oligosaccharides. At 168 h after inoculation the expression quantity (co-pies/2 μL) of PR-1a gene was increased to 2 469.6 in treated tobacco leaf, reached 392.6% than that at 0 h after inoculation,it was increased 3.05 times of that in untreated control.The activity change of defensive enzymes and PR-1a gene expression of tobacco （Nicotiana tabacum） seedling induced by chito-oligosaccharides were studied. The results showed that high level systemic acquired resistance （SAR） was expressed in tobacco plants treated with chito-oligosaccharides solution at the concentration of 50 μg/mL. PAL activity increased greatly with 2 peaks, the activity of SOD decreased initially followed by an increase with higher increment, and the activity of POD peaked early followed by a gentle fall in chito-oligosaccharide treated plants. The PR-1a gene was strongly expressed in tobacco due to systemic acquired resistance induced by chito-oligosaccharides. At 168 h after inoculation the expression quantity （co-pies/2 μL） of PR-1a gene was increased to 2 469.6 in treated tobacco leaf, reached 392.6% than that at 0 h after inoculation,it was increased 3.05 times of that in untreated control.

关 键 词：活性变化 防御酶系 壳寡糖 基因表达 烟草 诱导 系统获得抗病性 植物病毒 

分 类 号：S435.131.4[农业科学—农业昆虫与害虫防治] TS229[农业科学—植物保护]