Clonostachys rosea 67-1液生孢子形成及其抗性研究  

The sporulation of Clonostachys rosea strain 67-1 in submerged fermentation and its resistance to adversity

作　　者：张保元[1,2] 孙漫红[1] 张拥华[1] 谢响明[2] 李世东[1] 

机构地区：[1]中国农业科学院植物保护研究所,北京100081 [2]北京林业大学生物科学与技术学院,北京100083

出　　处：《植物病理学报》2010年第1期103-105,共3页Acta Phytopathologica Sinica

基　　金：国家"863"资助项目(2006AA10A211);国家"十一五"科技支撑计划项目(2006BAD08A02)

摘　　要：The growth and sporulation of Clonostachys rosea strain 67-1 in PD broth was observed and figured out. A large amount of submerged spores were obtained in shake flask with proprietary Czapek me-dium. Meanwhile, the colonies of strain 67-1were cultured in PDA plate and aerial spores were collected by elution. Resistances of submerged spores and aerial spores to high temperature, dry condition and UV treatment were determined. The results showed that the survival of the submerged spores kept in 60℃ for 30 min was 76.7%, while the aerial spores were hardly germinated in the same condition. After two weeks′ drying treatment, the spore activities were 89.2% and 29.3%, respectively. Similarly, the activity of submerged spores was 72.6% and that of aerial spores was 19.7% when exposing to UV for 1 min. It is illuminated that deep submerged fermentation is more efficient than solid culture for strain 67-1 to produce chlamydospores, which act as main component in biopesticide mass production.The growth and sporulation of Clonostachys rosea strain 67-1 in PD broth was observed and figured out. A large amount of submerged spores were obtained in shake flask with proprietary Czapek me-dium. Meanwhile, the colonies of strain 67-1were cultured in PDA plate and aerial spores were collected by elution. Resistances of submerged spores and aerial spores to high temperature, dry condition and UV treatment were determined. The results showed that the survival of the submerged spores kept in 60℃ for 30 min was 76.7%, while the aerial spores were hardly germinated in the same condition. After two weeks′ drying treatment, the spore activities were 89.2% and 29.3%, respectively. Similarly, the activity of submerged spores was 72.6% and that of aerial spores was 19.7% when exposing to UV for 1 min. It is illuminated that deep submerged fermentation is more efficient than solid culture for strain 67-1 to produce chlamydospores, which act as main component in biopesticide mass production.

关 键 词：孢子形成 粉红粘帚霉 抗性 拮抗微生物 重寄生菌 寄主范围 寄生能力 拮抗机制 

分 类 号：S646[农业科学—蔬菜学] S567.239[农业科学—园艺学]