光学成像技术在子宫内膜异位症动物模型中应用  

Current Applications in Animal Optical Imaging Technologies with Bioluminescence and Fluorescence in the Experimental Models of Endometriosis

作　　者：郑婷婷[1] 刘义[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院妇产科,武汉430022

出　　处：《国际妇产科学杂志》2010年第1期50-52,共3页Journal of International Obstetrics and Gynecology

基　　金：国家自然科学基金资助项目(30750007)

摘　　要：子宫内膜异位症(EMs)动物模型是研究人EMs的病因、发病机制和治疗方法的有效工具。在应用EMs动物模型的过程中,光学成像技术主要采用荧光与生物发光两种技术,可达到无创伤动态监测异位病灶形成和发展的目的。对目前运用绿色荧光蛋白(GFP)和羧基荧光素二乙酸盐琥珀酰亚胺酯(CFSE)等荧光标记技术和荧光素酶生物发光技术建立EMs动物模型,动态无损伤量化观察异位病灶的大小和生长情况的优缺点做文献综述。The experimental animal models as a xenographic host of induced endometriosis for relative biological behavior study of endometriosis have been developed and are of high value for the evaluation of the etiopathogenisis,pathogenesis and therapeutics underlying the development of this disease.This review describes these animal models of induced endometriosis using fluorescence in vivo imaging and bioluminescence to quantify the ectopic lesions of both size and number and assesses their different potentials and limitations in regard to endometriosis research,with the aim of developing novel non-invasive diagnostic tools.

关 键 词：光学成像技术 绿色荧光蛋白 荧光染料CFSE 荧光素酶 子宫内膜异位症 动物模型 

分 类 号：R711.71[医药卫生—妇产科学] R445[医药卫生—临床医学]