反相高效液相色谱法测定延肾胶囊中大黄素和大黄酚的含量  

Determination of Emodin and Chrysophanol in Yanshen Capsules by RP-HPLC

作　　者：曹健[1] 王芳[1] 雷健[1] 张卿[1] 张恩娟[1] 

机构地区：[1]第三军医大学第二临床医学院药学部,重庆市400037

出　　处：《中国医院用药评价与分析》2010年第2期148-149,共2页Evaluation and Analysis of Drug-use in Hospitals of China

摘　　要：目的:建立以反相高效液相色谱法测定延肾胶囊中大黄素及大黄酚含量的方法。方法:色谱柱为HypersilODS(150mm×4.6mm,10μm),流动相为甲醇-0.02mol.L-1磷酸(75:25),流速1.2m.lmin-1,检测波长438nm,柱温25℃。结果:大黄素在0.012～0.036mg.mL-1范围内线性关系良好(r=0.9997);平均加样回收率为100.2%(RSD=0.41%);大黄酚在0.0124～0.0372mg.mL-1范围内线性关系良好(r=0.9994);平均加样回收率为100.5%(RSD=2.22%)。结论:该方法简便易行,结果准确可靠,可适用于延肾胶囊的质量控制。OBJECTIVE：To establish a RP-HPLC method for determination of emodin and chysophanol in Yanshen Capsules. METHODS： The separation of samples was performed on HypersilODS （150 mm×4.6 mm,10 μm） with mobile phase composed of methanol-0.02% phosphoric acid solution （75： 25） at a flow rate of 1.2 mL·min^ -1 . The detection wavelength was set at 438 nm and the column temperature was kept at 25 ℃. RESULTS： The 1inear concentration ranges of emodin and chrysophanol were 0.012～0.036 mg·mL^ -1 （r=0.999 7） and 0.012 4 -0.037 2 mg·mL^ -1 （r= 0.999 4）,respectively,and their average recovery rates were 100.2% （RSD=0.41%） and 100.5% （RSD=2.22%）,respectively. CONCLUSION： The method is simple and accurate,and it is applicable for quality control of Yanshen Capsules.

关 键 词：反相高效液相色谱法 延肾胶囊 大黄素 大黄酚 含量测定 

分 类 号：R932[医药卫生—生药学]