GABA受体在静脉全麻药催眠作用的作用  

Effcets of bicuculline or securinine on the hypnotic effects of intravenous anesthetics

作　　者：许鹏程[1] 程伟[1] 马丽丽[1] 戴体俊[1] 

机构地区：[1]徐州医学院江苏省麻醉学重点实验室,221002

出　　处：《国际麻醉学与复苏杂志》2010年第1期13-15,共3页International Journal of Anesthesiology and Resuscitation

基　　金：国家自然科学基金资助项目（30872432）

摘　　要：目的探讨γ-氯基丁酸A（GABAA）受体与静脉全麻药戊巴比妥钠、羟丁酸钠、依托咪酯、丙泊酚和氯胺酮催眠作用的关系。方法建立小鼠腹腔注射静脉全麻药催眠模型，在催醒实验中分别观察侧脑室注射不同剂量的GABA。受体阻断药荷包牡丹碱（Bic）和静脉注射不同剂量的GABAA受体阻断药一叶秋碱（Se）对小鼠睡眠时间（sleepingtime，ST）的影响。结果Bic和Se能够剂量依赖性地缩短戊巴比妥钠、依托咪酯、丙泊酚和氯胺酮催眠小鼠的ST（P〈0．05或P〈0．01），但不能缩短羟丁酸钠催眠小鼠的ST。结论GABA、受体是戊巴比妥钠、依托咪酯、丙泊酚和氯胺酮催眠作用的重要靶位，可能不是羟丁酸钠催眠作用的靶位。Objective To investigate the relationship between GABAA receptor and the hypnotic effects of pentobarbital sodium, sodium hydroxybutyrate, etomidate, propofol and ketamine. Methods After having established the mice model of hypnosis by intraperitoneally injected appropriate doses of pentobarbital sodium, sodium hydroxybutyrate, etomidate, propofol or ketamine, we intracerebroventricularly or intravenously injected the mice with different doses of bicuculline （Bic） or securinine （Se）; and then observed effects on the sleeping time by using awaken test. Results Bic and Se significantly and dose-dependently decreased the sleeping time of mice treated with pentobarbital sodium, etomidate, propofol or ketaminc （P〈0.05, P〈0.01 ）, but had no distinctive effect on the sleeping time of mice treated with sodium hydroxybutyrate（P〉0.05 ）. Conclusion GABAA receptor may be important target for the hypnotic effect of pentobarbital sodium, etomidate, propofol and ketamine, but not be the targets for the hypnotic effects of sodium hydroxybutyrate.

关 键 词：GABAA 受体 静脉全麻药 荷包牡丹碱 一叶秋碱 催眠 催醒 

分 类 号：R614[医药卫生—麻醉学]