Biodegradation of Microcystin-RR by a New Isolated Sphingopyxis sp. USTB-05  

作　　者：王俊峰 吴鹏飞 陈建 闫海 

机构地区：[1]Department of Biological Science and Technology, School of Applied science, University of Science and Tech-nology Beijing, Beijing 100083, China [2]Department of Environmental Engineering, School of Resource and Environmental Engineering, Jiangxi Univer-sity of Science and Technology, Ganzhou 341000, China

出　　处：《Chinese Journal of Chemical Engineering》2010年第1期108-112,共5页中国化学工程学报（英文版）

基　　金：Supported by the State Key Development Program for Basic Research of China（2008CB418105）; the National Natural Science Foundation of China（203777008 20621703）; the State Key Joint Laboratory of Environment Simulation and Pollution Control（09K08ESPCT）; the Educational Committee of Beijing

摘　　要：A promising bacterial strain for the biodegradation of Microcystins(MCs)was isolated from Dianchi lake in China and identified as Sphingopyxis sp.USTB-05 by the analysis of 16s rDNA.Initial MC-RR of 42.3 mg·L -1 was completely degraded by USTB-05 within 36 h,which was a relatively high biodegradation rate of MC-RR.With the cell-free extract(CE)of Sphingopyxis sp.USTB-05,MC-RR was biodegraded at a more rapid biodegradation rate compared with its strain,so that initial MC-RR of 42.3 mg·L -1 was completely biodegraded within 10 h.During the bio-reaction of MC-RR catalyzed by CE,two intermediate metabolites and a dead-end product of MC-RR were observed on HPLC profiles and all of them had similar scanning profiles in the wavelength from 200 to 300 nm,indicating that the group of Adda in all products of MC-RR remained intact.A promising bacterial strain for the biodegradation of Microcystins（MCs）was isolated from Dianchi lake in China and identified as Sphingopyxis sp.USTB-05 by the analysis of 16s rDNA.Initial MC-RR of 42.3 mg·L -1 was completely degraded by USTB-05 within 36 h,which was a relatively high biodegradation rate of MC-RR.With the cell-free extract（CE）of Sphingopyxis sp.USTB-05,MC-RR was biodegraded at a more rapid biodegradation rate compared with its strain,so that initial MC-RR of 42.3 mg·L -1 was completely biodegraded within 10 h.During the bio-reaction of MC-RR catalyzed by CE,two intermediate metabolites and a dead-end product of MC-RR were observed on HPLC profiles and all of them had similar scanning profiles in the wavelength from 200 to 300 nm,indicating that the group of Adda in all products of MC-RR remained intact.

关 键 词：MICROCYSTIN-RR Sphingopyxis sp.USTB-05 cell-free extract BIODEGRADATION 

分 类 号：TQ324.9[化学工程—合成树脂塑料工业] G644[文化科学—高等教育学]