尖吻蝮蛇舌状虫粗抗原诊断价值的初步研究  

Preliminary evaluation on diagnostic value of raw antigen of Armillifer agkistrodontis nymph

作　　者：沈玉娟[1] 卢潍媛[1] 袁忠英[1] 徐馀信[1] 陈勤[1] 曹建平[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所,卫生部寄生虫病原与媒介生物学重点实验室,世界卫生组织疟疾、血吸虫病和丝虫病合作中心,上海200025

出　　处：《国际医学寄生虫病杂志》2010年第1期17-20,共4页International JOurnal of Medical Parasitic Diseases

基　　金：国家传染病重大专项（2008ZX10004-001,2008ZX10004-002,2009ZX10004-201）;中国疾病预防控制中心寄生虫病预防控制所中青年基金（Ipd200502）

摘　　要：目的建立一种快速、简便、灵敏和特异的舌形虫病血清学诊断方法。方法从人工感染尖吻蝮蛇舌状虫虫卵3个月的KM小鼠中分离尖吻蝮蛇舌状虫若虫，制备粗抗原，进行SDS—PAGE鉴定分析。用该粗抗原ELISA检测舌形虫病患者、不同寄生虫病患者和尖吻蝮蛇舌状虫实验感染小鼠血清特异性IgG抗体，其中舌形虫病患者血清1份，血吸虫病患者血清13份，囊尾蚴、并殖吸虫、肝吸虫和旋毛虫等病患者血清各5份，丝虫、裂头蚴病患者血清各1份，正常人血清10份以及舌形虫感染不同时间段的小鼠血清30份。结果舌形虫若虫粗抗原浓度为37．9mg／ml，SDS—PAGE鉴定分析，可见4条清晰的条带，相对分子质量为16000～150000。舌形虫病患者和29份舌形虫感染小鼠血清检查结果均为阳性，其他寄生虫病患者血清检查结果均为阴性。结论初步建立了基于尖吻蝮蛇舌状虫若虫粗抗原检测舌形虫病的ELISA方法，为该病临床诊断、疾病预防控制及试剂盒的研制提供依据和奠定了基础。Objective To establish a rapid, simple, sensitive and specific serodiagnostie method for pentastomiosis. Methods Nymphs were isolated from KM mice infected with eggs of Armillifer agkistrodontis for 3 months. The soluble raw antigens were prepared from the nymphs, analysed by SDS-PAGE, and were used for detecting specific IgG antibodies in sera from patients with different parasitic diseases wherein 1 with pentastomiosis, 13 with schistosomiasis, 5 with eystieereosis, paragonimiasis, elonorchiasis and trichinosis, respectively, 1 with filariasis, 1 with pleroeereoidnsis, 10 healthy persons and 30 KM mice infected with A. agkistrodontis at different time by ELISA. Results The concentration of soluble raw antigens was 37.9 mg/ ml, and 4 different bands about Mr 16 000-150 000 were visible on SDS-PAGE gels. The sera of pentastomiosis and 29 KM mice infected with A. agkistrodontis were positive, and the others were negative by ELISA detection. Conclusion ELISA was established for diagnosing pentastomiosis using soluble raw antigens of A. agkistrodontis nymphs.

关 键 词：舌形虫病 尖吻蝮蛇舌状虫 粗抗原 免疫诊断 

分 类 号：R53[医药卫生—内科学] R996.3[医药卫生—临床医学]