猪2-细胞胚胎电融合  

ELECTROFUSION OF 2 CELL PIG EMBRYOS

作　　者：赵浩斌[1] 陈乃清[2] 魏庆信[1] 

机构地区：[1]湖北省农业科学院畜牧兽医研究所,武汉430209 [2]西北农业大学动物科学系,咸阳712100

出　　处：《华中农业大学学报》1998年第6期558-562,共5页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金;湖北省自然科学基金资助项目

摘　　要：以湖北白猪为试验动物 ,研究了不同处理对猪 2 细胞胚胎电融合的影响 ,结果表明 :融合液的离子强度、电场强度、脉冲时程和交流电处理等均影响电融合的效率 ,以非电解质的甘露醇溶液为融合液 ,融合前交流脉冲处理 ,直流电场强度为 150 0～ 2 0 0 0V/cm和脉冲时程为 4 0～ 60 μs时可获得较高的融合效率。The effects of different treatments on electrofusion of pig 2 cell embryos were studied with Hubei white pigs as experimental animals. The results showed that electrolyte, electric field strength, pulse duration, and AC treatment before DC pulse would affect the electrofusion efficiency. The highest electrofusion efficiency could be obtained if the fusion medium was mannitol solution, AC treatment was adopted before DC pulse, the DC electric field strength was 1?500～2?000 V/cm, and the pulse duration was 40～60 μs.

关 键 词：猪 2-细胞胚胎 电融合 胚胎 细胞融合 

分 类 号：S828.2[农业科学—畜牧学]