玉米转座因子Ac在转基因烟草及其后代中的转座行为  

Transposition and Behavior of the Maize Element Ac in Nicotiana tabacum L.

作　　者：瞿绍洪[1,2] 李银心[1,2] 景建康[1,2] 胡含 朱至清[1,2] 张文俊 

机构地区：[1]中国科学院遗传研究所植物细胞与染色体工程国家重点实验室 [2]中国科学院植物研究所

出　　处：《农业生物技术学报》1998年第4期313-317,共5页Journal of Agricultural Biotechnology

摘　　要：用带嵌合基因３５Ｓ－Ａｃ－ＧＵＳ的农杆菌双元载体转化普通烟草品种Ｎｃ８９，对转基因植株（Ｔ０）及其回交后代（ＢＣＦ１）进行ＧＵＳ组织化学分析，检测到转座因子Ａｃ在部分Ｔ０代和ＢＣＦ１代植株中转座。用Ａｃ作探针，与上述Ｔ０代和ＢＣＦ１代植株的基因组ＤＮＡ进行Ｓｏｕｔｈｅｒｎ杂交，结果表明，Ａｃ从原来所在的Ｔ－ＤＮＡ解离，并整合到烟草基因组的其它位点。同时对Ａｃ－２植株Ｔ１代的ＮＰＴＩＩ基因和Ａｃ因子进行多重ＰＣＲ分析，发现Ａｃ因子与ＮＰＴＩ基因间出现遗传重组，也间接证明Ａｃ发生了转座。最后还对转座因子标签研究中转座基因型及突变体筛选的策略进行了讨论。The maize transposable element Activator was transformed into Nicotiana tabacum L. with Agrobacterium tumefaciens harboring the chimeric gene 35S Ac GUS. Transgenic plants and their offsprings were examined by GUS (β glucuronidase) histochemical assay. Different transposition patterns of Ac element were observed in the tobacco somatic tissues. Multiple PCR with selfed progenies of the transgenic plant Ac 2 revealed that genetic recombination occurred between Ac and NPTII gene (including T DNA). The genomic DNAs of transgenic plants and their offsprings were probed with Ac and GUS fragments in Southern blot analysis. The results indicated that Ac transposed in T 0 and T 1 generations. A strategy for the selection of transposition genotypes was discussed.

关 键 词：Ac转座因子 烟草 转座因子标签 突变体 筛选 

分 类 号：S188[农业科学—农业基础科学] S572