重组乙型肝炎疫苗(中国仓鼠卵巢细胞)免疫12年的效果研究  

12 Years Immunological Effects of Hepatitis B Vaccine Made by Recombinant Deoxyribonucleic Acid Techniques in Chinese Hamster Ovary Cell

作　　者：齐顺祥[1] 张新江[2] 张建立[2] 张志勇[2] 郝志勇[2] 陈吉朝[2] 褚娟[2] 马景臣[2] 赵玉良[1] 王锋[3] 张勇[3] 毕胜利[3] 

机构地区：[1]河北省疾病预防控制中心,石家庄050021 [2]正定县疾病预防控制中心,河北正定050800 [3]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室,北京100052

出　　处：《中国疫苗和免疫》2010年第1期1-4,共4页Chinese Journal of Vaccines and Immunization

基　　金：国家十一.五科学技术重大专项"我国乙型病毒性肝炎免疫预防策略研究"(编号:2008ZX10002-001)

摘　　要：目的研究1997～1999年出生人群,接种重组乙型肝炎(乙肝)疫苗[Hepatitis B Vaccine Made by Recombinant Deoxyribonucleic Acid(DNA)Techniques in Chinese Hamster Ovary(CHO)Cell,HepB(CHO)]的免疫效果。方法将正定县1997～1999年出生人群接种重组HepB(CHO)的三个乡作为研究现场,以户籍登记在册的全部儿童为研究对象,核实重组HepB(CHO)接种史,采集静脉血5ml,无菌分离血清,使用固相放射免疫试剂检测乙肝病毒表面抗原[Hepatitis B Virus(HBV)Surface Antigen,HBsAg]和抗乙肝病毒核心抗原抗体(Anti-HBV Core Antibody,Anti-HBc);抗乙肝病毒表面抗原抗体(Anti-HBV Surface Antibody,Anti-HBs)使用Abbott公司生产的微粒子酶免疫测定(Microparticle Enzyme Immunoassay,MEIA)试剂定量检测。结果免疫后10～12年的Anti-HBs阳性率分别为71.0%、72.1%、68.2%,平均70.3%;Anti-HBs的几何平均浓度(Geometric Mean Concentration,GMC)分别为197.9mIU/ml(毫国际单位/毫升)、296.0mIU/ml、158.0mIU/ml,平均207.9mIU/ml。HBsAg阳性率为0.50%,Anti-HBc阳性率为2.26%。结论重组HepB(CHO)免疫后10～12年效果良好。Objevtive To understand the long-term immunological effects of a recombined hepatitis B vaccine [HepB made by recombinant deoxyribonucleic acid （DNA）techniques in chinese hamster ovary （CHO）cell, HepB（CHO）]. Method from 1997 to 1999 all newborns in Zhengding County were administered to HepB （CHO） at birth. Children in 3 of all 7 towns in the county were selected as study objects to cheek their hepatitis B vaccination history and test for HBV markers. Results Children who aged 10, 11 and 12 years after immunization, the anti-HBs positive rates were 71.0%0,72.1% and 68.2% respectively, and the geometric mean concentration （GMCs）were 197.9mIU/ml, 296.0mIU/ml and 158.0mIU/ml respectively; Anti-HBs GMCs were 197.9mIU/ml, 296.0mIU/ml and 158.0mIU/ml respectively. The Average GMC was 207.9mIU/ml. HBsAg and anti-HBc positive rates were 0.50% and 2.26 % respectively. Conclusion The long-term immunological effects of HepB （CHO） are excellent.

关 键 词：乙型病毒性肝炎 重组乙型肝炎疫苗(中国仓鼠卵巢细胞) 免疫持久性 

分 类 号：R186[医药卫生—流行病学] R512.62[医药卫生—公共卫生与预防医学]