河北省急性弛缓性麻痹病例中脊髓灰质炎病毒VP_1编码区基因特征分析  

Gene Characteristic of VP_1 Region of Poliovirus Isolates from Acute Flaccid Paralysis in Hebei Province

作　　者：陈玫[1] 严冬梅[2] 朱晖[2] 郭彧[1] 张俊棉[1] 张振国[1] 赵娜[1] 崔志强[1] 许文波[2] 

机构地区：[1]河北省疾病预防控制中心,石家庄050021 [2]中国疾病预防控制中心病毒病预防控制所病毒基因工程国家重点实验室,世界卫生组织西太平洋区脊髓灰质炎参比实验室,北京100050

出　　处：《中国疫苗和免疫》2010年第1期33-37,共5页Chinese Journal of Vaccines and Immunization

摘　　要：目的对河北省2007～2008年急性弛缓性麻痹(Acute Flaccid Paralysis,AFP)病例和密切接触者的粪便标本中,分离的脊髓灰质炎(脊灰)病毒进行VP1编码区基因特征分析,为维持无脊灰提供依据。方法按世界卫生组织《脊灰实验室手册》进行病毒分离与血清定型,用逆转录-聚合酶链反应扩增VP1编码区基因片段,并进行核苷酸序列测定和分析,构建基因亲缘关系树。结果河北省2007～2008年,从AFP病例和密切接触者粪便标本中共分离到脊灰病毒42株,以Ⅱ、Ⅲ型血清型为主,均为疫苗类似株,未发现野病毒和疫苗衍生脊灰病毒。结论脊灰病毒VP1编码区基因特征分析可用于识别脊灰病毒传播途径,并对免疫规划工作具有指导作用。Objective In order to provide the evidence for polio free status, the VP1 gene characteristic analysis of poliovirus isolated from acute flaccid paralysis （AFP）cases and close contacts were conducted in Hebei Province during 2007-2008. Methods Virus isolation and identification were conducted according to the 4th edition of WHO polio laboratory manual. The sequence of VP1 region were amplified and sequenced. The phylogenetic trees based on VP1 region were constructed. Result The total number of poliovirus isolates were 42. Most isolates were type Ⅱ and Ⅲ. No wild poliovirus and vaccine derived poliovirus （VDPVs） was detected. Conclusion Gene characteristic of VP1 region of poliovirus can be used to identify the polio virus transmission and guide the work of immunization program.

关 键 词：脊髓灰质炎病毒 VP1编码区 核苷酸序列测定和分析 

分 类 号：R373.22[医药卫生—病原生物学]