毛细管电泳指纹图谱应用于叶下珠药材中黄酮的检测  

Application of Capillary Electrophoretic Finger Print Atlas to the Determination of Flavones in the TCM of Phyllanthus Urinarial

作　　者：邓光辉[1,2] 胡炜[2] 农林[1] 潘丽娟[1] 张桂华[1] 

机构地区：[1]广西民族大学化学与生态工程学院,南宁530006 [2]广西大学化学化工学院,南宁530004

出　　处：《理化检验（化学分册）》2010年第2期113-116,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：广西自然科学基金项目(桂科自:0640038)资助

摘　　要：建立了叶下珠药材中黄酮的毛细管电泳指纹图谱。以硼砂和十二烷基磺酸钠(pH9.0)为背景电解质溶液,运行电压15kV,紫外检测波长245nm,流体静压力进样15s(高度12cm),对不同产地叶下珠药材进样检测。按电泳峰共有率fi≥70%为依据,确定10个不同产地叶下珠药材中黄酮的毛细管电泳指纹峰为8个,各产地叶下珠的毛细管电泳指纹图谱与标准毛细管电泳指纹图谱的相似度较好。在制备供试液后不同时间进样测定,各指纹峰的相对迁移时间的相对标准偏差小于5%,相对峰面积的相对标准偏差在3.0%～7.8%之间,结果表明样品在48h内稳定。The electrophoretic finger print atlas of flavones in the TCM of phyllanthus urinarial was established by capillary electrophoresis. A mixture of 60 mmol · L^-1 borax solution and 16 mmol · L^-1 sodium dodecyl sufate solution （adjusted to pH 9 with 0. 1 mmol · L^-1 boric acid solution preliminarily） was used as background electrolyte, and moving voltage of 15 kV was applied. UV-detection at the wavelength of 245 um was adopted- Samples were introduced hydrostatically at a height of 12 cm for 15 s. Samples of phyllanthus urinarial grown in 10 different areas were taken for analysis. Base on the value of rate of common electrophoretic peaks fi≥ 70%, a set of electrophoretic finger print atlas of 8 peaks was determined for flavones in phyllanthus urinarial samples from 10 different areas in our country. Good similarities between the eleetrophoretie finger print atlases of the samples and the standards were obtained. Relative standard deviation among the values of relative migration times of various finger print peaks obtained at different time of sample introduction was less than 5 %, and values of RSD＇s for the relative peak areas were in the range from 3. 0% to 7. 8%. The results obtained proved that the sample was stable within 48h.

关 键 词：毛细管电泳 叶下珠 黄酮 电泳指纹图谱 

分 类 号：O657.9[理学—分析化学]