虎杖发酵液中白藜芦醇的分离纯化工艺研究  

Isolation and purification of resveratrol from fermentation broth of Polygonum cuspidatum

作　　者：王辉[1,2] 董悦生[2] 秦建全[2] 王亚俊[2] 修志龙[2] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海南海口571101 [2]大连理工大学环境与生命学院生物科学与工程系,辽宁大连116024

出　　处：《中草药》2010年第2期223-227,共5页Chinese Traditional and Herbal Drugs

基　　金：教育部新世纪优秀人才基金资助项目(NCET-05-0279)

摘　　要：目的分离纯化虎杖发酵液中的白藜芦醇。方法利用大孔树脂结合聚酰胺柱色谱从虎杖发酵液的提取液中纯化白藜芦醇,并研究了大孔树脂和聚酰胺对白藜芦醇的吸附动力学。结果大孔树脂和聚酰胺对白藜芦醇的表观静态吸附量分别为11.70 mg/g和57.9 mg/g,表观动态吸附量分别为12.47 mg/g和58.98 mg/g,吸附行为符合Langmuir动力学。利用大孔树脂结合聚酰胺两步柱色谱纯化提取液中的白藜芦醇,使其纯度达到95.8%,总收率达69.8%。结论此方法操作简单,成本低廉,白藜芦醇的纯度和收率高,有工业化应用前景。Objective To isolate and purify resveratrol from fermentation broth of Polygonum cuspidatum. Methods Macroporous resin and polyamide column chromatography were combined to purify resveratrol from the extract in fermentation broth of P. cuspidatum, and the adsorption dynamics of resveratrol was evaluated. Results Apparent static adsorptive capacities of resveratrol on macroporous resin and polyamide were 11.70 and 57.9 mg/g, respectively, and apparent dynamic adsorptive capacities were 12.47 and 58. 98 mg/g,respectively. Both of the adsorption isotherms correlate well with Langmuir type. After macroporous resin and polyamide two-step column chromatography, the purity of resveratrol reached 95.8%, with the yield of 69.8%. Conclusion It is proved the method is potential for industrial application due to its simple procedure, lower cost as well as high purity and yield of resveratrol.

关 键 词：虎杖 白藜芦醇 大孔树脂 聚酰胺 

分 类 号：R286.02[医药卫生—中药学]